User:Elaine Marie Robbins/Notebook/CHEM-496/2012/02/14: Difference between revisions

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==Objective==
==Objective==


To continue the experiment performed on [[User:Elaine Marie Robbins/Notebook/CHEM-496/2012/02/01| 02/01/12]] to determine the effects of various concentrations of tris buffer on AuNP absorbance.


==Description==
==Description==


# Take UV-vis data for various dilutions of tris buffer (pH 10.9)
#* 1 M
#* 500 mM
#* 200 mM
#* 100 mM
#* 75 mM


==Data==


==Data==
* Graph of absorbance vs wavelength for AuNP and different dilutions of tris
 
<center>[[Image:Absorbance vs wavelength magnified.jpg]]</center>
 
While all concentrations of tris buffer cause a peak shift towards shorter wavelengths, the higher concentrations tended to yield lower absorbance values.
 
 
* Graph of peak absorbance vs -log(ionic strength of tris buffer solution).
 
<center>[[Image:Peak absorbance vs -log(ionic strength) 2-14-12.jpg]]</center>
 
 
* Graph of peak wavelength vs -log(ionic strength of tris buffer solution).


<center>[[Image:Peak wavelength vs -log(ionic strength) 2-14-12.jpg]]</center>


==Notes==
==Notes==

Revision as of 12:50, 21 February 2012

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Objective

To continue the experiment performed on 02/01/12 to determine the effects of various concentrations of tris buffer on AuNP absorbance.

Description

  1. Take UV-vis data for various dilutions of tris buffer (pH 10.9)
    • 1 M
    • 500 mM
    • 200 mM
    • 100 mM
    • 75 mM

Data

  • Graph of absorbance vs wavelength for AuNP and different dilutions of tris

While all concentrations of tris buffer cause a peak shift towards shorter wavelengths, the higher concentrations tended to yield lower absorbance values.


  • Graph of peak absorbance vs -log(ionic strength of tris buffer solution).


  • Graph of peak wavelength vs -log(ionic strength of tris buffer solution).

Notes



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