User:Elaine Marie Robbins/Notebook/CHEM-496/2012/02/15: Difference between revisions

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==Description==
==Description==


<b>Synthesizing AuNP</b>


# Combine 2 mL of 3.66 mM HAuCl4 and 6.04 mL of 0.0173 mM BSA in a test tube.
# Dilute to 20 mL with water.
# Place in oven at 74°C.
# Leave solutions in oven for 3.5 hr.
# Continue to take UV-vis data of 1 mL 70 Au/BSA ratio solution synthesized on [[User:Elaine Marie Robbins/Notebook/CHEM-496/2012/01/31| 01/31/12]] over time.
<b>Determining the effect of tris buffer on 166 Au/BSA ratio solution</b>
# Take UV-vis data of 166 Au/BSA ratio solution synthesized on [[User:Elaine Marie Robbins/Notebook/CHEM-496/2012/01/31| 01/31/12]].
# Add 2 mL 50 mM tris buffer (pH 10.1) to solid protein fibers (no solution).
# Allow fibers to settle.
# Take UV-vis data of tris/fiber solution.


==Data==
==Data==

Revision as of 12:08, 15 February 2012

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Objective

Description

Synthesizing AuNP

  1. Combine 2 mL of 3.66 mM HAuCl4 and 6.04 mL of 0.0173 mM BSA in a test tube.
  2. Dilute to 20 mL with water.
  3. Place in oven at 74°C.
  4. Leave solutions in oven for 3.5 hr.
  5. Continue to take UV-vis data of 1 mL 70 Au/BSA ratio solution synthesized on 01/31/12 over time.

Determining the effect of tris buffer on 166 Au/BSA ratio solution

  1. Take UV-vis data of 166 Au/BSA ratio solution synthesized on 01/31/12.
  2. Add 2 mL 50 mM tris buffer (pH 10.1) to solid protein fibers (no solution).
  3. Allow fibers to settle.
  4. Take UV-vis data of tris/fiber solution.

Data

  • Graph of absorbance vs wavelength of the AuNP solution synthesized on 01/31/12 over time.


  • Graph of the peak absorbance value versus time of the AuNP solution synthesized on 01/31/12.

Over time, absorbance values increase dramatically.


  • Graph of the wavelength at which the peak absorbance was recorded versus time.

Over time, the optimum wavelength tends to get shorter.

Notes



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