User:Elaine Marie Robbins/Notebook/CHEM-496/2012/02/15: Difference between revisions
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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Effect of Tris Buffer on Protein Fibers</span> | ||
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==Objective== | ==Objective== | ||
To synthesize a solution of AuNP, to continue to take UV-vis data over time of 70 Au/BSA solution synthesized on [[User:Elaine Marie Robbins/Notebook/CHEM-496/2012/01/31 |01/31/11]], and to determine the effect of tris buffer on protein fibers also synthesized on [[User:Elaine Marie Robbins/Notebook/CHEM-496/2012/01/31 |01/31/11]]. | |||
==Description== | ==Description== | ||
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<b>Synthesizing AuNP</b> | <b>Synthesizing AuNP</b> | ||
# Combine 2 mL of 3.66 mM | # Combine 2 mL of 3.66 mM HAuCl<sub>4</sub> and 6.04 mL of 0.0173 mM BSA in a test tube. | ||
# Dilute to 20 mL with water. | # Dilute to 20 mL with water. | ||
# Place in oven at | # Place in oven at 80°C. | ||
# Leave solutions in oven for 3 | # Leave solutions in oven for 3 hr. | ||
# Continue to take UV-vis data of 1 mL 70 Au/BSA ratio solution synthesized on [[User:Elaine Marie Robbins/Notebook/CHEM-496/2012/01/31| 01/31/12]] over time. | # Continue to take UV-vis data of 1 mL 70 Au/BSA ratio solution synthesized on [[User:Elaine Marie Robbins/Notebook/CHEM-496/2012/01/31| 01/31/12]] over time. | ||
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Over time, the optimum wavelength tends to get shorter. | Over time, the optimum wavelength tends to get shorter. | ||
* Graph of absorbance vs wavelength of the protein fibers with added 50 mM tris buffer (pH 10.1) | |||
<center>[[Image:Absorbance vs wavelength tris 2-15-12.jpg]]</center> | |||
* AuNP solution: | |||
<center>[[Image:Aunp solution.JPG]]</center> | |||
==Notes== | ==Notes== | ||
Revision as of 11:13, 21 February 2012
 Effect of Tris Buffer on Protein Fibers
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ObjectiveTo synthesize a solution of AuNP, to continue to take UV-vis data over time of 70 Au/BSA solution synthesized on 01/31/11, and to determine the effect of tris buffer on protein fibers also synthesized on 01/31/11. DescriptionSynthesizing AuNP
Data
 Over time, absorbance values increase dramatically.
 Over time, the optimum wavelength tends to get shorter.
 Notes
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