User:Elaine Marie Robbins/Notebook/CHEM-496/2012/02/21
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Effect of Tris Buffer on Protein Fibers II | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
ObjectiveTo take UV-vis data of tris + 50 mM tris buffer (pH 10.1) mixed on 02/15/12, and to synthesize protein fibers. DescriptionSynthesizing AuNP
Data
High absorbance values obtained from sample prior to centrifuging is most likely due to light scattering caused by solid protein fibers.
NotesThe presence of a peak at 500-550 nm after centrifuging indicates that AuNP may be present in solution, even though the solution is a 166 Au/BSA ratio solution. Prior to the addition of the 50 mM tris solution (pH 10.1), all Au particles were associated with the protein fibers, and none were in solution.
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