User:Elaine Marie Robbins/Notebook/CHEM-496/2012/02/21

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Effect of Tris Buffer on Protein Fibers II <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Objective

To take UV-vis data of tris + 50 mM tris buffer (pH 10.1) mixed on 02/12/12, and to synthesize protein fibers.

Description

Synthesizing AuNP

  1. Combine 1 mL of 3.66 mM HAuCl4 and 1.27 mL of 0.0173 mM BSA in a test tube.
  2. Dilute to 10 mL with water.
  3. Place in oven at 75°C.
  4. Leave solutions in oven for 3 hr.


Determining the effect of tris buffer on 166 Au/BSA ratio solution

  1. Take UV-vis data of 166 Au/BSA ratio solution synthesized on 01/31/12 and modified on 02/15/12.
  2. Centrifuge for 10 min at 13,200 rpm
  3. Take UV-vis data of supernatant.

Data

Notes



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