User:Elaine Marie Robbins/Notebook/CHEM-496/2012/02/28: Difference between revisions
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|style="background-color: # | |style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Effect of Tris Buffer on Protein Fibers III</span> | ||
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | |||
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==Objective== | |||
To take UV-vis data of tris + 50 mM tris buffer (pH 10.1) mixed on [[User:Elaine Marie Robbins/Notebook/CHEM-496/2012/02/15 |02/15/12]]. | |||
==Description== | |||
# Centrifuge AuNP fibers + 50 mM tris solution made on [[User:Elaine Marie Robbins/Notebook/CHEM-496/2012/02/15 |02/15/12]] at 13200 rpm for 10 min. | |||
# Take UV-vis absorption data. | |||
# Compare data to that obtained on [[User:Elaine Marie Robbins/Notebook/CHEM-496/2012/02/15 |02/15/12]] and [[User:Elaine Marie Robbins/Notebook/CHEM-496/2012/02/21 |02/21/12]]. | |||
== | ==Data== | ||
* | |||
* Graph of absorbance vs wavelength over time for the AuNP fibers + 50 mM tris after centrifuge: | |||
<center>[[Image:Absorbance vs wavelength magnified 2-28-12.png]]</center> | |||
Overall absorbance decreased this week, however it appears that the peak wavelength has continued to shift lightly towards shorter wavelengths. | |||
==Notes== | ==Notes== | ||
[[Category:Course]] | [[Category:Course]] | ||
[[Category:Miscellaneous]] | [[Category:Miscellaneous]] | ||
Revision as of 10:39, 7 March 2012
Effect of Tris Buffer on Protein Fibers III | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
ObjectiveTo take UV-vis data of tris + 50 mM tris buffer (pH 10.1) mixed on 02/15/12. Description
Data
Overall absorbance decreased this week, however it appears that the peak wavelength has continued to shift lightly towards shorter wavelengths. Notes
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