User:Elaine Marie Robbins/Notebook/CHEM-496/2012/02/28: Difference between revisions

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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Effect of Tris Buffer on Protein Fibers III</span>
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==Objective==


To take UV-vis data of tris + 50 mM tris buffer (pH 10.1) mixed on [[User:Elaine Marie Robbins/Notebook/CHEM-496/2012/02/15 |02/15/12]].


==Description==


# Centrifuge AuNP fibers + 50 mM tris solution made on [[User:Elaine Marie Robbins/Notebook/CHEM-496/2012/02/15 |02/15/12]] at 13200 rpm for 10 min.
# Take UV-vis absorption data.
# Compare data to that obtained on [[User:Elaine Marie Robbins/Notebook/CHEM-496/2012/02/15 |02/15/12]] and [[User:Elaine Marie Robbins/Notebook/CHEM-496/2012/02/21 |02/21/12]].


==Objective==
==Data==
*Add your objective here
 
stuff [[User:Matt Hartings|Matt Hartings]] 21:41, 6 September 2011 (EDT)
* Graph of absorbance vs wavelength over time for the AuNP fibers + 50 mM tris after centrifuge:


==Description==
<center>[[Image:Absorbance vs wavelength magnified 2-28-12.png]]</center>
*Add description here
more stuff [[User:Matt Hartings|Matt Hartings]] 21:43, 6 September 2011 (EDT)


==Data==
Overall absorbance decreased this week, however it appears that the peak wavelength has continued to shift lightly towards shorter wavelengths.
* Add data and results here...


==Notes==
==Notes==
This area is for any observations or conclusions that you would like to note.
Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.


[[Category:Course]]
[[Category:Course]]
[[Category:Miscellaneous]]
[[Category:Miscellaneous]]





Revision as of 10:39, 7 March 2012

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Objective

To take UV-vis data of tris + 50 mM tris buffer (pH 10.1) mixed on 02/15/12.

Description

  1. Centrifuge AuNP fibers + 50 mM tris solution made on 02/15/12 at 13200 rpm for 10 min.
  2. Take UV-vis absorption data.
  3. Compare data to that obtained on 02/15/12 and 02/21/12.

Data

  • Graph of absorbance vs wavelength over time for the AuNP fibers + 50 mM tris after centrifuge:

Overall absorbance decreased this week, however it appears that the peak wavelength has continued to shift lightly towards shorter wavelengths.

Notes