User:Elaine Marie Robbins/Notebook/CHEM-496/2012/03/06: Difference between revisions

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==Description==
==Description==
<b>Effect of Au/BSA Ratio</b>


# Make solutions with the following Au/BSA mole ratios using 0.1 mL HAuCl<sub>4</sub>:
# Make solutions with the following Au/BSA mole ratios using 0.1 mL HAuCl<sub>4</sub>:
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# Place in oven at 80°C.  
# Place in oven at 80°C.  
# Leave solutions in oven for 3.5 hr.
# Leave solutions in oven for 3.5 hr.
# Centrifuge solutions for 10 min at 13200 rpm.
# Take UV-vis data of supernatant.
<b>Effect of Tris Buffer on Protein Fibers</b>
# Centrifuge AuNP fibers + 50 mM tris solution made on [[User:Elaine Marie Robbins/Notebook/CHEM-496/2012/02/15 |02/15/12]].
# Take UV-vis absorption data.
# Compare data to that obtained on [[User:Elaine Marie Robbins/Notebook/CHEM-496/2012/02/15 |02/15/12]], [[User:Elaine Marie Robbins/Notebook/CHEM-496/2012/02/21 |02/21/12]], and [[User:Elaine Marie Robbins/Notebook/CHEM-496/2012/02/28 |02/28/12]].


==Data==
==Data==


<b>Effect of Au/BSA Ratio</b>
* 90 Au/BSA Ratio
<center>[[Image:90 ratio.JPG]]</center>
* 110 Au/BSA Ratio
<center>[[Image:110 ratio.JPG]]</center>
* 130 Au/BSA Ratio
<center>[[Image:130 ratio.JPG]]</center>


* 150 Au/BSA Ratio
<center>[[Image:150 ratio.JPG]]</center>
* Graph of absorbance vs wavelength for each Au/BSA ratio:
<center>[[Image:Avsorbance vs wavelength (magnified).png]]</center>
The transition from AuNP solution to AuNP/BSA fibers occurs between 130 Au/BSA and 150 Au/BSA.
<b>Effect of Tris Buffer on Protein Fibers</b>
* Graphs of absorbance vs wavelength over time of the AuNP fibers and 50 mM tris (pH 10.1) solution made on [[User:Elaine Marie Robbins/Notebook/CHEM-496/2012/02/15 |02/15/12]]:
<center>[[Image:Absorbance vs wavelength over time 3-6-12.png]]</center>
<center>[[Image:Absorbance vs wavelength over time magnified 3-6-12.png]]</center>


==Notes==
==Notes==
<b>Effect of Au/BSA Ratio</b>
After centrifuge, all four solutions had at least some solid present in the solution. Only the 150 Au/BSA solution had a significant amount of visible fiber formation.


[[Category:Course]]
[[Category:Course]]

Revision as of 12:38, 7 March 2012

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Objective

To determine the effect of the mole ratio of Au/BSA on AuNP synthesis.

Description

Effect of Au/BSA Ratio

  1. Make solutions with the following Au/BSA mole ratios using 0.1 mL HAuCl4:
    • 90 Au/BSA
    • 110 Au/BSA
    • 130 Au/BSA
    • 150 Au/BSA
  2. Dilute to 1 mL with water.
  3. Place in oven at 80°C.
  4. Leave solutions in oven for 3.5 hr.
  5. Centrifuge solutions for 10 min at 13200 rpm.
  6. Take UV-vis data of supernatant.


Effect of Tris Buffer on Protein Fibers

  1. Centrifuge AuNP fibers + 50 mM tris solution made on 02/15/12.
  2. Take UV-vis absorption data.
  3. Compare data to that obtained on 02/15/12, 02/21/12, and 02/28/12.

Data

Effect of Au/BSA Ratio

  • 90 Au/BSA Ratio
  • 110 Au/BSA Ratio
  • 130 Au/BSA Ratio
  • 150 Au/BSA Ratio
  • Graph of absorbance vs wavelength for each Au/BSA ratio:

The transition from AuNP solution to AuNP/BSA fibers occurs between 130 Au/BSA and 150 Au/BSA.


Effect of Tris Buffer on Protein Fibers

  • Graphs of absorbance vs wavelength over time of the AuNP fibers and 50 mM tris (pH 10.1) solution made on 02/15/12:

Notes

Effect of Au/BSA Ratio

After centrifuge, all four solutions had at least some solid present in the solution. Only the 150 Au/BSA solution had a significant amount of visible fiber formation.



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