User:Elaine Marie Robbins/Notebook/CHEM-496/2012/03/07: Difference between revisions

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==Description==
==Description==
<b>Effect of Au/BSA Ratio</b>


# Make solutions with the following Au/BSA mole ratios using 0.1 mL HAuCl<sub>4</sub>:
# Make solutions with the following Au/BSA mole ratios using 0.1 mL HAuCl<sub>4</sub>:
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#* 144 Au/BSA
#* 144 Au/BSA
#* 146 Au/BSA
#* 146 Au/BSA
#* 148 Au/BSA
# Dilute to 1 mL with water.
# Dilute to 1 mL with water.
# Place in oven at 80°C.  
# Place in oven at 80°C.  

Revision as of 12:29, 9 March 2012

Au/BSA Mole Ratios II <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Objective

To determine the effect of the mole ratio of Au/BSA on AuNP synthesis.

Description

  1. Make solutions with the following Au/BSA mole ratios using 0.1 mL HAuCl4:
    • 132 Au/BSA
    • 134 Au/BSA
    • 136 Au/BSA
    • 138 Au/BSA
    • 140 Au/BSA
    • 142 Au/BSA
    • 144 Au/BSA
    • 146 Au/BSA
  2. Dilute to 1 mL with water.
  3. Place in oven at 80°C.
  4. Leave solutions in oven for 3 hr.
  5. Centrifuge solutions at 13200 rpm for 10 min.
  6. Take UV-vis data of supernatants.

Data

  • Graph of absorbance vs wavelength for each Au/BSA ratio:

Between 132 Au/BSA and 134 Au/BSA, AuNP solution stops forming, and protein fibers begin to form.

Notes



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