User:Elaine Marie Robbins/Notebook/CHEM-496/2012/04/04: Difference between revisions
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==Description== | ==Description== | ||
# Add 40 μL of BSA or AuNP solution (synthesized on [[User:Elaine Marie Robbins/Notebook/CHEM-496/2012/02/15 |02/15/12]])to 40 μL 4 M NaOH. | # Add 40 μL of BSA or AuNP solution (synthesized on [[User:Elaine Marie Robbins/Notebook/CHEM-496/2012/02/15 |02/15/12]]) to 40 μL 4 M NaOH. | ||
# Add 2μL 450 mM DTT. | # Add 2μL 450 mM DTT. | ||
# Heat in microwave for 1 min. | # Heat in microwave for 1 min. | ||
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==Data== | ==Data== | ||
* Graph of UV-vis data: | |||
<center>[[Image:Absorbance vs wavelength 4-4-12.png]]</center> | |||
==Notes== | ==Notes== | ||
Revision as of 09:40, 17 April 2012
 Microwave-Assisted Base Hydrolysis
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ObjectiveTo test the effects of microwave-assisted base hydrolysis to determine the concentration of free BSA in solution. Description
Data
 Notes
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