User:Elaine Marie Robbins/Notebook/Capstone/2012/06/14

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Fluorescence of MB Open and Closed <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Methods

  1. Take fluorescence data of the MB, the complementary strand, and both together.
  2. Make solutions containing either citrate-capped or BSA-conjugated AuNP and various cell dilutions:
    1. Mix 3 μL AuNP/TS primer, 1 μL 50x dNTP mix, 16 μL sterile water, 2.5 μL 10x PCR buffer, and 2 μL cell extract in a PCR tube.
      • Cell dilutions used: 400000, 200000, 100000, 50000, 25000.
    2. Incubate in heat block at 30°C for 30 min.
    3. Add 6.10 μL molecular beacon.
    4. Incubate in heat block at 37°C for 1 hr.
    5. Take fluorescence spectra.

Data

  • Fluorescence of MB open and closed, excited at 515 nm, 10 nm slit width:


  • Fluorescence of solutions containing citrate-capped AuNP, excited at 515 nm:


  • Peak fluorescence intensity vs. number of cells for citrate-capped AuNP:


  • Fluorescence of solutions containing BSA-conjugated AuNP, excited at 515 nm:


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