User:Elaine Marie Robbins/Notebook/Capstone/2012/07/12

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Fluorescence of Open MB</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Entry title==
 
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* Insert content here...
 
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==Methods==
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# Make 3 solutions of MB [MB] = 10 nM:
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#* MB alone
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#* MB + complement, complement:MB = 10:1
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#* MB + complement, complement:MB = 1:1
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# Heat in heat block at 75°C for 30 min.
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# Incubate at room temperature for 30 min.
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# Run fluorescence.
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==Data==
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Revision as of 09:44, 12 July 2012

Fluorescence of Open MB Main project page
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Methods

  1. Make 3 solutions of MB [MB] = 10 nM:
    • MB alone
    • MB + complement, complement:MB = 10:1
    • MB + complement, complement:MB = 1:1
  2. Heat in heat block at 75°C for 30 min.
  3. Incubate at room temperature for 30 min.
  4. Run fluorescence.

Data


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