User:Eleni N. Kalivas/Notebook/CHEM-571/2013/08/28: Difference between revisions
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==Citrate-AuNP== | ==Citrate-AuNP== | ||
Protocol: | |||
*Take 50mL of the HAuCl4 solution from the 250mL volumetric flask, with a .249mM concentration | |||
*Heat this solution to boiling while stirring | |||
*Add 1.5mL of 1% (w/v) sodium citrate | |||
*Boil solution for another 40 minutes | |||
*Cool to room temperature and measure the volume | |||
*Determine the final concentration of gold and citrate | |||
Revision as of 13:37, 28 August 2013
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ObjectivesBSA-AuNPProtocol: 1. Add 1mL of the 2.54mM gold (HAuCl4) solution to a 10mL volumetric flask 2. Add 1.8mL of BSA solution so that the final concentration of gold is 90X that of BSA 3. Add deionized water up to 10mL 4. Transfer solution to a test tube and cap with aluminum foil 5. Heat in oven at 80C for 3 hours 6. Transfer solution to a plastic falcon tube
Citrate-AuNPProtocol:
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