User:Eleni N. Kalivas/Notebook/CHEM-571/2013/09/04: Difference between revisions
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== | ==Overview== | ||
Due to time constraints we were not able to run a UV-vis for the inosine spectra. The objective for today includes analyzing yesterdays data in order to check the spectra and calibration curves and make any necessary corrections. Also to finish running the inosine absorbance spectra. | |||
==Analysis== | |||
After looking over the data from yesterday there were multiple problems that needed correcting. First we noticed that our 0.5M adenosine was the exact same data as the water that was run as a blank. This lead us to believe that the data was saved incorrectly and that some of the dilutions may have just been off. We re-ran the 0.5M adenosine sample and got comparatively valid data. We then noticed that our 1.0M adenosine peaked below the 0.5M which indicated a clear error. Therefore we re-made the 1.0M adenosine and received much better data. Our last observation was that the 3.0M adenosine was too far off when analyzing the calibration curve, so we also re-made the 3.0M adenosine dilution and re-ran it and received much better data. | |||
==Inosine== | |||
Once the Adenosine samples had been run and the spectra and the calibration curve had been corrected, the inosine samples were now being run. After running all the data the spectra was plotted and the calibration curve was made. Analysis of the calibration curve concluded | |||
Revision as of 11:20, 4 September 2013
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OverviewDue to time constraints we were not able to run a UV-vis for the inosine spectra. The objective for today includes analyzing yesterdays data in order to check the spectra and calibration curves and make any necessary corrections. Also to finish running the inosine absorbance spectra. AnalysisAfter looking over the data from yesterday there were multiple problems that needed correcting. First we noticed that our 0.5M adenosine was the exact same data as the water that was run as a blank. This lead us to believe that the data was saved incorrectly and that some of the dilutions may have just been off. We re-ran the 0.5M adenosine sample and got comparatively valid data. We then noticed that our 1.0M adenosine peaked below the 0.5M which indicated a clear error. Therefore we re-made the 1.0M adenosine and received much better data. Our last observation was that the 3.0M adenosine was too far off when analyzing the calibration curve, so we also re-made the 3.0M adenosine dilution and re-ran it and received much better data. InosineOnce the Adenosine samples had been run and the spectra and the calibration curve had been corrected, the inosine samples were now being run. After running all the data the spectra was plotted and the calibration curve was made. Analysis of the calibration curve concluded
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