User:Eleni N. Kalivas/Notebook/CHEM-571/2013/09/11: Difference between revisions

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*Based on our calibration curve we were able to determine the concentration of the citrate gold nano particles, which then allows us to figure out the size of the diameter which can allow us to determine how many AuNP we can determine how many nanoparticles are present.  
*Based on our calibration curve we were able to determine the concentration of the citrate gold nano particles, which then allows us to figure out the size of the diameter which can allow us to determine how many AuNP we can determine how many nanoparticles are present.  


[[Image:Eleni Kalivas Tables Grubbs Test.png]]


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Revision as of 10:29, 18 September 2013

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Objective

Bradford Assay

Figure 1: Absorption spectra of ADA standard solutions prepared by Bradford Assay Trial #4

  • Today the blank was manually subtracted from the absorbance data which yielded the correct shape graph, Also the samples this time were much darker blue then the samples from Tuesday, September 10th.

Figure 2: Calibration Curve for ADA standard solutions at 596nm

Atomic Absorption of standards

  • based off the standards prepared yesterday the atomic absorption of our standards were tested and a calibration curve was created based on these values:

  • This data was then combined with the data of each group in order to construct a class calibration curve:

  • Based on our calibration curve we were able to determine the concentration of the citrate gold nano particles, which then allows us to figure out the size of the diameter which can allow us to determine how many AuNP we can determine how many nanoparticles are present.


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