User:Eleni N. Kalivas/Notebook/CHEM-571/2013/09/11

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Atomic Absorption of standards)
(Atomic Absorption of standards)
Line 6: Line 6:
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
 +
==Objective==
 +
 +
==Bradford Assay==
 +
Figure 1: Absorption spectra of ADA standard solutions prepared by Bradford Assay Trial #4
 +
[[Image:BRADFORDASSAY TRAIL-4 ZEMENK.png]]
 +
 +
*Today the blank was manually subtracted from the absorbance data which yielded the correct shape graph, Also the samples this time were much darker blue then the samples from Tuesday, September 10th.
 +
 +
Figure 2:  Calibration Curve for ADA standard solutions at 596nm
 +
[[Image:Calibration Curve ADA ZEM-0911-zemenk.png]]
 +
==Atomic Absorption of standards==
==Atomic Absorption of standards==
* based off the standards prepared yesterday the atomic absorption of our standards were tested and a calibration curve was created based on these values:
* based off the standards prepared yesterday the atomic absorption of our standards were tested and a calibration curve was created based on these values:
Line 13: Line 24:
*This data was then combined with the data of each group in order to construct a class calibration curve:
*This data was then combined with the data of each group in order to construct a class calibration curve:
-
[[]]
+
[[Image:Kalivas Eleni group calibration data.png]]
*Based on our calibration curve we were able to determine the concentration of the citrate gold nano particles, which then allows us to figure out the size of the diameter which can allow us to determine how many AuNP we can determine how many nanoparticles are present.  
*Based on our calibration curve we were able to determine the concentration of the citrate gold nano particles, which then allows us to figure out the size of the diameter which can allow us to determine how many AuNP we can determine how many nanoparticles are present.  

Revision as of 13:27, 18 September 2013

Project name Main project page
Previous entry      Next entry

Objective

Bradford Assay

Figure 1: Absorption spectra of ADA standard solutions prepared by Bradford Assay Trial #4 Image:BRADFORDASSAY TRAIL-4 ZEMENK.png

  • Today the blank was manually subtracted from the absorbance data which yielded the correct shape graph, Also the samples this time were much darker blue then the samples from Tuesday, September 10th.

Figure 2: Calibration Curve for ADA standard solutions at 596nm Image:Calibration Curve ADA ZEM-0911-zemenk.png

Atomic Absorption of standards

  • based off the standards prepared yesterday the atomic absorption of our standards were tested and a calibration curve was created based on these values:

  • This data was then combined with the data of each group in order to construct a class calibration curve:

Image:Kalivas Eleni group calibration data.png

  • Based on our calibration curve we were able to determine the concentration of the citrate gold nano particles, which then allows us to figure out the size of the diameter which can allow us to determine how many AuNP we can determine how many nanoparticles are present.



Personal tools