User:Eleni N. Kalivas/Notebook/CHEM-571/2013/09/11: Difference between revisions
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* Run BSA solution prepared on August, 28th on the AA. | * Run BSA solution prepared on August, 28th on the AA. | ||
* Prepare a 1/10 dilution of the Au-citrate solution prepared August, 28th and run this sample on the AA. | * Prepare a 1/10 dilution of the Au-citrate solution prepared August, 28th and run this sample on the AA. | ||
* Prepare calibration curve for | * Prepare calibration curve for AuNP solutions and determine the concentration of Au in the BSA and citrate solutions. | ||
* Re-run bradford assay of adenosine deaminase that was unsuccessful on september 10th. | * Re-run bradford assay of adenosine deaminase that was unsuccessful on september 10th. | ||
* Determine number of atoms of gold in the BSA and citrate gold solutions using the prepared gold calibration curve and the diameter size of the gold particles in the BSA and citrate solutions(this can be determined from the UV/vis samples run of these on August 28th. | * Determine number of atoms of gold in the BSA and citrate gold solutions using the prepared gold calibration curve and the diameter size of the gold particles in the BSA and citrate solutions(this can be determined from the UV/vis samples run of these on August 28th. |
Revision as of 10:34, 18 September 2013
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Objective
Bradford AssayFigure 1: Absorption spectra of ADA standard solutions prepared by Bradford Assay Trial #4
Figure 2: Calibration Curve for ADA standard solutions at 596nm Atomic Absorption of standards
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