User:Eleni N. Kalivas/Notebook/CHEM-571/2013/09/17: Difference between revisions
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*Take UV/vis spectrum of the sample. | *Take UV/vis spectrum of the sample. | ||
*Repeat steps 4-5 until complete oxidation of HRP. | *Repeat steps 4-5 until complete oxidation of HRP. | ||
[[Image:Eleni Kalivas Tables For 091713 zemenk.png]] | |||
==UV/vis Spectra== | |||
Revision as of 10:41, 18 September 2013
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ObjectiveThe objective of today's lab was to determine the amount of reagent necessary to fully reduce horseradish peroxidase (HRP). HRP oxidation will be done with potassium ferricyanide K3[Fe(CN)6]. K3[Fe(CN)6], which has a reduction potential of 424mV. HRP reduction will be done with Sodium Dithionite.
HRP OxidationHRP Oxidation Protocol
UV/vis Spectra |