User:Eleni N. Kalivas/Notebook/CHEM-571/2013/09/17: Difference between revisions
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==Objective== | ==Objective== | ||
The objective of today's lab was to determine the amount of reagent necessary to fully reduce horseradish peroxidase (HRP). HRP oxidation will be done with potassium ferricyanide K<sub>3</sub>[Fe(CN)<sub>6</sub>]. K<sub>3</sub>[Fe(CN)<sub>6</sub>], which has a reduction potential of 424mV. HRP reduction will be done | The objective of today's lab was to determine the amount of reagent necessary to fully reduce horseradish peroxidase (HRP). HRP oxidation will be done with potassium ferricyanide K<sub>3</sub>[Fe(CN)<sub>6</sub>]. K<sub>3</sub>[Fe(CN)<sub>6</sub>], which has a reduction potential of 424mV. HRP reduction will be done with Sodium Dithionite. | ||
* the goal is to determine the endpoints of the oxidation and reduction of HRP. | |||
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Revision as of 10:36, 18 September 2013
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ObjectiveThe objective of today's lab was to determine the amount of reagent necessary to fully reduce horseradish peroxidase (HRP). HRP oxidation will be done with potassium ferricyanide K3[Fe(CN)6]. K3[Fe(CN)6], which has a reduction potential of 424mV. HRP reduction will be done with Sodium Dithionite.
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