User:Eleni N. Kalivas/Notebook/CHEM-571/2013/09/17: Difference between revisions
From OpenWetWare
Line 10: | Line 10: | ||
* the goal is to determine the endpoints of the oxidation and reduction of HRP. | * the goal is to determine the endpoints of the oxidation and reduction of HRP. | ||
=HRP Oxidation= | |||
'''HRP Oxidation Protocol''' | '''HRP Oxidation Protocol''' | ||
*Run UV-vis of a blank buffer sample. | *Run UV-vis of a blank buffer sample. |
Revision as of 10:48, 18 September 2013
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
ObjectiveThe objective of today's lab was to determine the amount of reagent necessary to fully reduce horseradish peroxidase (HRP). HRP oxidation will be done with potassium ferricyanide K3[Fe(CN)6]. K3[Fe(CN)6], which has a reduction potential of 424mV. HRP reduction will be done with Sodium Dithionite.
HRP OxidationHRP Oxidation Protocol
UV/vis SpectraFigure 1: Normalized Absorption Spectra of the Oxidation of HRP with K3[Fe(CN)6] Figure 2: Enlarged Absorption Spectra of the Oxidation of HRP with K3[Fe(CN)6]
|