User:Eleni N. Kalivas/Notebook/CHEM-571/2013/09/18: Difference between revisions

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*Equation used to calculate the percent oxidized was:
 
(Abs.partially oxidized 402.44nm-Abs. fully reduced 402.44nm)/(Abs.fully oxidized-Abs.fully reduced)*100
<br>




Revision as of 11:25, 24 September 2013

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Objective

  • The objective for today is to perform a redox titration on HRP in order to determine the standard redox potential of the protein.

Redox Titration

  • The protocol for this lab was found here.

Specific volume of samples used during redox titration.

Sample number Volume of HRP Sample(mL)
1 1
2 1.05
3 1.055
4 1.056
5 1.059
6 1.064
7 1.074
8 1.084

Absorbance Spectra of the redox titration of HRP


Redox titration of HRP (percent oxidized)

% Oxidized Potential
-2.633063185 -0.5122
0 -0.5803
3.260060544 -0.4781
30.97191805 -0.4045
82.70859316 0.0146
98.04493512 -0.3879
98.62977455 -0.355
100 -0.3519






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