User:Eleni N. Kalivas/Notebook/CHEM-571/2013/09/18

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(Redox Titration)
(Redox Titration)
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==Redox Titration==
==Redox Titration==
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*The protocol for this lab was found [[User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2013/09/18|here]].
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Specific volume of samples used during redox titration.
-
Specific volume of samples used during redox titration.
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{| {{table}}
{| {{table}}
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| 8||1.084
| 8||1.084
|}
|}
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 +
Absorbance Spectra of the redox titration of HRP
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[[Image:PercentreducedHRPzem.png]]
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 +
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Redox titration of HRP (percent oxidized)
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''% Oxidized'''
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| align="center" style="background:#f0f0f0;"|'''Potential'''
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|-
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| -2.633063185||-0.5122
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|-
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| 0||-0.5803
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|-
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| 3.260060544||-0.4781
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|-
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| 30.97191805||-0.4045
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|-
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| 82.70859316||0.0146
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|-
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| 98.04493512||-0.3879
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|-
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| 98.62977455||-0.355
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|-
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| 100||-0.3519
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|}
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*Equation used to calculate the percent oxidized was:
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(Abs.partially oxidized 402.44nm-Abs. fully reduced 402.44nm)/(Abs.fully oxidized-Abs.fully reduced)*100
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<br>

Revision as of 12:50, 24 September 2013

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Objective

  • The objective for today is to perform a redox titration on HRP in order to determine the standard redox potential of the protein.

Redox Titration

  • The protocol for this lab was found here.

Specific volume of samples used during redox titration.

Sample number Volume of HRP Sample(mL)
11
21.05
31.055
41.056
51.059
61.064
71.074
81.084

Absorbance Spectra of the redox titration of HRP Image:PercentreducedHRPzem.png


Redox titration of HRP (percent oxidized)

% Oxidized Potential
-2.633063185-0.5122
0-0.5803
3.260060544-0.4781
30.97191805-0.4045
82.708593160.0146
98.04493512-0.3879
98.62977455-0.355
100-0.3519
  • Equation used to calculate the percent oxidized was:

(Abs.partially oxidized 402.44nm-Abs. fully reduced 402.44nm)/(Abs.fully oxidized-Abs.fully reduced)*100





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