User:Eleni N. Kalivas/Notebook/CHEM-571/2013/09/18: Difference between revisions

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==Redox Titration==
==Redox Titration==
*The protocol for this lab was found [[User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2013/09/18|here]].


 
Specific volume of samples used during redox titration.
Specific volume of samples used during redox titration.


{| {{table}}
{| {{table}}
Line 34: Line 34:
| 8||1.084
| 8||1.084
|}
|}
Absorbance Spectra of the redox titration of HRP
[[Image:PercentreducedHRPzem.png]]
Redox titration of HRP (percent oxidized)
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''% Oxidized'''
| align="center" style="background:#f0f0f0;"|'''Potential'''
|-
| -2.633063185||-0.5122
|-
| 0||-0.5803
|-
| 3.260060544||-0.4781
|-
| 30.97191805||-0.4045
|-
| 82.70859316||0.0146
|-
| 98.04493512||-0.3879
|-
| 98.62977455||-0.355
|-
| 100||-0.3519
|}
*Equation used to calculate the percent oxidized was:
(Abs.partially oxidized 402.44nm-Abs. fully reduced 402.44nm)/(Abs.fully oxidized-Abs.fully reduced)*100
<br>




Revision as of 10:50, 24 September 2013

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Objective

  • The objective for today is to perform a redox titration on HRP in order to determine the standard redox potential of the protein.

Redox Titration

  • The protocol for this lab was found here.

Specific volume of samples used during redox titration.

Sample number Volume of HRP Sample(mL)
1 1
2 1.05
3 1.055
4 1.056
5 1.059
6 1.064
7 1.074
8 1.084

Absorbance Spectra of the redox titration of HRP


Redox titration of HRP (percent oxidized)

% Oxidized Potential
-2.633063185 -0.5122
0 -0.5803
3.260060544 -0.4781
30.97191805 -0.4045
82.70859316 0.0146
98.04493512 -0.3879
98.62977455 -0.355
100 -0.3519
  • Equation used to calculate the percent oxidized was:

(Abs.partially oxidized 402.44nm-Abs. fully reduced 402.44nm)/(Abs.fully oxidized-Abs.fully reduced)*100





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