User:Eleni N. Kalivas/Notebook/CHEM-571/2013/09/18: Difference between revisions

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Absorbance Spectra of the redox titration of HRP
Absorbance Spectra of the redox titration of HRP
[[Image:FullyoxidizedreducedHRPzem.png]]
Potential of Redox titration of HRP
[[Image:PercentreducedHRPzem.png]]
[[Image:PercentreducedHRPzem.png]]




Redox titration of HRP (percent oxidized)
Redox Titration of HRP


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Revision as of 10:08, 25 September 2013

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Objective

  • The objective for today is to perform a redox titration on HRP in order to determine the standard redox potential of the protein.

Redox Titration

  • The protocol for this lab was found here.

Specific volume of samples used during redox titration.

Sample number Volume of HRP Sample(mL)
1 1
2 1.05
3 1.055
4 1.056
5 1.059
6 1.064
7 1.074
8 1.084

Absorbance Spectra of the redox titration of HRP


Potential of Redox titration of HRP


Redox Titration of HRP

% Oxidized Potential
-2.633063185 -0.5122
0 -0.5803
3.260060544 -0.4781
30.97191805 -0.4045
82.70859316 0.0146
98.04493512 -0.3879
98.62977455 -0.355
100 -0.3519






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