User:Eleni N. Kalivas/Notebook/CHEM-571/2013/09/24: Difference between revisions
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==Protocol== | ==Protocol== | ||
The following protocol was taken from [User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2013/09/24 Matt Hartings] | The following protocol was taken from [[User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2013/09/24|Matt Hartings]] | ||
We will allow the pepsin cleavage of hemoglobin to proceed for two hours with aliquots taken and analyzed every 1/2 hour. We will also run parallel reactions with different concentrations of pepstatin (pepsin inhibitor) present. We will save our aliquots for analysis tomorrow using SDS-PAGE. | We will allow the pepsin cleavage of hemoglobin to proceed for two hours with aliquots taken and analyzed every 1/2 hour. We will also run parallel reactions with different concentrations of pepstatin (pepsin inhibitor) present. We will save our aliquots for analysis tomorrow using SDS-PAGE. |
Revision as of 09:52, 24 September 2013
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ProtocolThe following protocol was taken from Matt Hartings We will allow the pepsin cleavage of hemoglobin to proceed for two hours with aliquots taken and analyzed every 1/2 hour. We will also run parallel reactions with different concentrations of pepstatin (pepsin inhibitor) present. We will save our aliquots for analysis tomorrow using SDS-PAGE.
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