User:Eleni N. Kalivas/Notebook/CHEM-571/2013/09/25: Difference between revisions
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#*Place gel in Destain Solution (10% acetic acid, 90% water) for 15 minutes | #*Place gel in Destain Solution (10% acetic acid, 90% water) for 15 minutes | ||
#Repeat last part of step 4 twice. | #Repeat last part of step 4 twice. | ||
Sample Set-up of the electrophoresis (SDS-PAGE) | Sample Set-up of the electrophoresis (SDS-PAGE) | ||
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<br> | <br> | ||
Image of SDS Electrophoresis | |||
[[Image:FLUBBER.jpg|750px]] | |||
==UV vis== | |||
Corrected Absorbance Spectra of Pepsin with Hemoglobin | |||
[[Image:Hemopepsin09251013zem.png|750px]] | |||
NOTES: For the 2 hour sample some pieces of protein may have been present when running the sample. | |||
Corrected Absorbance Spectra of 20μM Pepstatin with Hemoglobin | |||
[[Image:Pepstathemogl09252013zem.png|750px]] | |||
Revision as of 11:30, 9 October 2013
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Objective
ProtocolGeneral Protocol:
UV visCorrected Absorbance Spectra of Pepsin with Hemoglobin NOTES: For the 2 hour sample some pieces of protein may have been present when running the sample. Corrected Absorbance Spectra of 20μM Pepstatin with Hemoglobin
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