User:Eleni N. Kalivas/Notebook/CHEM-571/2013/10/08: Difference between revisions
From OpenWetWare
(Autocreate 2013/10/08 Entry for User:Eleni_N._Kalivas/Notebook/CHEM-571) |
|||
Line 6: | Line 6: | ||
| colspan="2"| | | colspan="2"| | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
== | ==Objective== | ||
The objective of today's lab is to observe and measure ADA turnover kinetics in the absence of an inhibitor. The results from this experimentation will be used in comparison to our ADA-AuNP turnover studies. | |||
==Protocol== | |||
The following protocol was taken from [[User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2013/10/08|Matt Harting's]] notebook | |||
# Make a 40μM solution of adenosine in buffer (50mM phosphate buffer, pH 7.4) | |||
# Go to Dr. Hartings lab for enzyme kinetics measurements. | |||
## Add 3mL of adenosine solution to the cuvette | |||
## Start your kinetics measurement | |||
### 1ms integration (on front panel) | |||
### 10 scan average (on front panel) | |||
### Set "Save the first available scan every" to 15 seconds (after clicking File>Save) | |||
### Set "Stop after this amount of time" to 10 minutes (after clicking File>Save) | |||
### Set "File Type" to Tab Delimited | |||
### Give the files a directory and a name | |||
### Click accept | |||
### Just before 1 minute add 30ul of 0.01u/mL ADA | |||
Revision as of 10:15, 8 October 2013
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
ObjectiveThe objective of today's lab is to observe and measure ADA turnover kinetics in the absence of an inhibitor. The results from this experimentation will be used in comparison to our ADA-AuNP turnover studies. ProtocolThe following protocol was taken from Matt Harting's notebook
|