User:Eleni N. Kalivas/Notebook/CHEM-571/2013/10/09: Difference between revisions
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(Autocreate 2013/10/09 Entry for User:Eleni_N._Kalivas/Notebook/CHEM-571) |
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== | ==Objective== | ||
The objective of today's lab is to observe and measure ADA turnover kinetics in the presence of, EHNA, an inhibitor. | |||
==Protocol== | |||
The following protocol was taken from [[User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2013/10/08|Matt Harting's]] notebook | |||
# Make a new 40μM solution of adenosine in buffer (50mM phosphate buffer, pH 7.4) | |||
# Go to Dr. Hartings lab for enzyme kinetics measurements. | |||
## Add 2μL of EHNA to the 40μM solution of adenosine buffer | |||
## Add 3mL of adenosine solution to the cuvette | |||
## Start your kinetics measurement | |||
### 1ms integration (on front panel) | |||
### 10 scan average (on front panel) | |||
### Set "Save the first available scan every" to 15 seconds (after clicking File>Save) | |||
### Set "Stop after this amount of time" to 10 minutes (after clicking File>Save) | |||
### Set "File Type" to Tab Delimited | |||
### Give the files a directory and a name | |||
### Click accept | |||
### Just before 1 minute add 30ul of 1.1u/mL ADA | |||
==Data== | |||
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Revision as of 11:27, 9 October 2013
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ObjectiveThe objective of today's lab is to observe and measure ADA turnover kinetics in the presence of, EHNA, an inhibitor. ProtocolThe following protocol was taken from Matt Harting's notebook
Data |