User:Eleni N. Kalivas/Notebook/CHEM-571/2013/10/22: Difference between revisions
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*Absorbance of BSA-AuNPs was determined for future comparison of the activity of protein-AuNPs. | *Absorbance of BSA-AuNPs was determined for future comparison of the activity of protein-AuNPs. | ||
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Absorbance spectra of a 1/10 dilution of BSA-AuNPs | |||
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[[Image:BSAAuNPsdilutionzem.png]] | [[Image:BSAAuNPsdilutionzem.png]] | ||
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*Absorbance of all starting materials used for synthesis of AuNPs today for future comparison | *Absorbance of all starting materials used for synthesis of AuNPs today for future comparison | ||
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Absorbance spectra of a 1/10 dilution of 2.52mM gold in water | |||
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[[Image:Golddilutionzem.png]] | [[Image:Golddilutionzem.png]] | ||
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Absorbance spectra of 1/2 dilution of 15.3μM HRP | |||
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[[Image:HalfdilutedHRP10222013zem.png]] | [[Image:HalfdilutedHRP10222013zem.png]] | ||
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Absorbance spectrum of 1/10 dilution of 15.6μM pepsin | |||
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[[Image:Pepsindilutionzem.png]] | [[Image:Pepsindilutionzem.png]] |
Revision as of 14:24, 29 October 2013
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ObjectiveThe objective of today's lab is to synthesize AuNPs for reactivity tests tomorrow. Also to determine the Au;Protein ratio that is required for fiber formation of HRP and for pepsin. ProtocolThe following has been taken from Matt Harting's lab notebook.
HRP-AuNP and Pepsin-AuNP
Data
Absorbance spectra of a 1/10 dilution of BSA-AuNPs
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