Objective
The objective of today's lab is to synthesize AuNPs for reactivity tests tomorrow. Also to determine the Au;Protein ratio that is required for fiber formation of HRP and for pepsin.
Protocol
The following has been taken from Matt Harting's lab notebook.
- Synthesis of HRP-AuNPs and Pepsin-AuNPs
- Into test tube that will contain a total of 5mL add
- Au solution such that the final concentration is 0.25mM
- Protein solution such that the final concentration reflects the following Au:Protein ratios
- 30
- 50
- 70
- 90
- 110
- 130
- 150
- 170
- 190
- 210
- Water up to 5mL
HRP-AuNP and Pepsin-AuNP
Table 1. Preparation of Protein-AuNP solutions
| Ratio
|
Amount of gold added(mL)
|
concentration HRP(M)
|
concentration Pepsin(M)
|
Volume HRP(mL)
|
Volume Pepsin(mL)
|
Volume Water HRP (mL)
|
Volume Water Pepsin (mL)
|
| 30 |
0.496031746 |
8.33333E-06 |
8.33333E-06 |
2.723311547 |
2.653927813 |
1.780656707 |
1.850040441
|
| 50 |
0.496031746 |
0.000005 |
0.000005 |
1.633986928 |
1.592356688 |
2.869981326 |
2.911611566
|
| 70 |
0.496031746 |
3.57143E-06 |
3.57143E-06 |
1.16713352 |
1.137397634 |
3.336834734 |
3.36657062
|
| 90 |
0.496031746 |
2.77778E-06 |
2.77778E-06 |
0.907770516 |
0.884642604 |
3.596197738 |
3.61932565
|
| 110 |
0.496031746 |
2.27273E-06 |
2.27273E-06 |
0.742721331 |
0.723798494 |
3.761246923 |
3.780169759
|
| 130 |
0.496031746 |
1.92308E-06 |
1.92308E-06 |
0.628456511 |
0.61244488 |
3.875511743 |
3.891523374
|
| 150 |
0.496031746 |
1.66667E-06 |
1.66667E-06 |
0.544662309 |
0.530785563 |
3.959305945 |
3.973182691
|
| 170 |
0.496031746 |
1.47059E-06 |
1.47059E-06 |
0.480584391 |
0.468340202 |
4.023383863 |
4.035628052
|
| 190 |
0.496031746 |
1.31579E-06 |
1.31579E-06 |
0.42999656 |
0.419041234 |
4.073971694 |
4.08492702
|
| 210 |
0.496031746 |
1.19048E-06 |
1.19048E-06 |
0.389044507 |
0.379132545 |
4.114923747 |
4.124835709
|
| 
Project name
