User:Eleni N. Kalivas/Notebook/CHEM-571/2013/10/23: Difference between revisions
From OpenWetWare
(→Data) |
|||
Line 74: | Line 74: | ||
## Water up to 5mL | ## Water up to 5mL | ||
== | ==HRP-AuNP and Myoglobin-AuNP== | ||
*The protein-AuNP solutions prepared on [[User:Moira M. Esson/Notebook/CHEM-571/2013/10/22|10/22/2013]] did not produce fibers. As such, new protein-AuNP solutions with increased ratios of protein-AuNP were prepared in order to determine the optimal concentration of Au to protein to synthesize fibers. | |||
*The general protocol for the synthesis of HRP-AuNP and Pepsin-AuNP follows the general protocol for the preparation of protein wrapped Au-NPs described on [[User:Moira M. Esson/Notebook/CHEM-571/2013/08/28|08/28/2013]]. | |||
*5mL of each solution was prepared. | |||
<br> | |||
Table 1. Stock solutions used in the preparation of Protein-AuNP solutions | |||
<br> | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Concentration HRP Day 2(M)''' | |||
| align="center" style="background:#f0f0f0;"|'''Concentration Gold Stock (M)''' | |||
| align="center" style="background:#f0f0f0;"|'''Myoglobin (M)''' | |||
|- | |||
| 0.0000015||0.0025||0.0000165 | |||
|} | |||
<br> | |||
Table 2. Preparation of HRP-AuNP solutions | |||
<br> | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Ratio''' | |||
| align="center" style="background:#f0f0f0;"|'''Amount of gold added (mL)''' | |||
| align="center" style="background:#f0f0f0;"|'''Concentration HRP (M)''' | |||
| align="center" style="background:#f0f0f0;"|'''Volume HRP (mL)''' | |||
| align="center" style="background:#f0f0f0;"|'''Volume H<sub>2</sub>O added (mL)''' | |||
|- | |||
| 230||0.5||1.08696E-06||3.623188406||0.876811594 | |||
|- | |||
| 250||0.5||0.000001||3.333333333||1.166666667 | |||
|- | |||
| 270||0.5||9.25926E-07||3.086419753||1.413580247 | |||
|- | |||
| 290||0.5||8.62069E-07||2.873563218||1.626436782 | |||
|- | |||
| 310||0.5||8.06452E-07||2.688172043||1.811827957 | |||
|- | |||
| 330||0.5||7.57576E-07||2.525252525||1.974747475 | |||
|- | |||
| 350||0.5||7.14286E-07||2.380952381||2.119047619 | |||
|- | |||
| 370||0.5||6.75676E-07||2.252252252||2.247747748 | |||
|- | |||
| 390||0.5||6.41026E-07||2.136752137||2.363247863 | |||
|- | |||
| 410||0.5||6.09756E-07||2.032520325||2.467479675 | |||
|} | |||
<br> | |||
Table 3. Preparation of Myoglobin-AuNPs | |||
<br> | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Ratio''' | |||
| align="center" style="background:#f0f0f0;"|'''Amount of gold added (mL)''' | |||
| align="center" style="background:#f0f0f0;"|'''Concentration Myoglobin (M)''' | |||
| align="center" style="background:#f0f0f0;"|'''Volume of myogloblin(mL)''' | |||
| align="center" style="background:#f0f0f0;"|'''Volume Water Myoglobin(mL)''' | |||
|- | |||
| 30||0.5||8.33333E-06||2.525252525||1.974747475 | |||
|- | |||
| 50||0.5||0.000005||1.515151515||2.984848485 | |||
|- | |||
| 70||0.5||3.57143E-06||1.082251082||3.417748918 | |||
|- | |||
| 90||0.5||2.77778E-06||0.841750842||3.658249158 | |||
|- | |||
| 110||0.5||2.27273E-06||0.688705234||3.811294766 | |||
|- | |||
| 130||0.5||1.92308E-06||0.582750583||3.917249417 | |||
|- | |||
| 150||0.5||1.66667E-06||0.505050505||3.994949495 | |||
|- | |||
| 170||0.5||1.47059E-06||0.445632799||4.054367201 | |||
|- | |||
| 190||0.5||1.31579E-06||0.398724083||4.101275917 | |||
|- | |||
| 210||1.19048E-06||0.360750361||4.139249639 | |||
|} | |||
<br> | |||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> |
Revision as of 14:26, 29 October 2013
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
ObjectiveTo take spectra of all 20 samples from Yesterday. Today we will create 10 new samples of the same Au:protein ratio, using myoglobin. Also, since no fibers formed 10 new samples will be prepared with a higher concentration. Lastly, we will be measuring pepsin activity of pepsin in solution, and also of pepsin-AuNPs in solution. ProtocolThe following protocol was taken from Matt Hartings notebook. Make a 37C water bath that can hold at least 3 test tubes (same size as you used yesterday) at a time --- START THIS FIRST
HRP-AuNP and Myoglobin-AuNP
|