User:Eleni N. Kalivas/Notebook/CHEM-571/2013/10/23: Difference between revisions
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Corrected Absorbance spectra of pepsin-AuNPs | Corrected Absorbance spectra of pepsin-AuNPs | ||
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[[Image:Pepsin Nano.png]] | [[Image:Pepsin Nano.png|750 px]] | ||
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*Both 30:1 and 70:1 pepsin-AuNP had an absorbance peak at 518nm. | *Both 30:1 and 70:1 pepsin-AuNP had an absorbance peak at 518nm. |
Revision as of 14:28, 29 October 2013
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
ObjectiveTo take spectra of all 20 samples from Yesterday. Today we will create 10 new samples of the same Au:protein ratio, using myoglobin. Also, since no fibers formed 10 new samples will be prepared with a higher concentration. Lastly, we will be measuring pepsin activity of pepsin in solution, and also of pepsin-AuNPs in solution. ProtocolThe following protocol was taken from Matt Hartings notebook. Make a 37C water bath that can hold at least 3 test tubes (same size as you used yesterday) at a time --- START THIS FIRST
HRP-AuNP and Myoglobin-AuNP
DataCorrected Absorbance spectra of pepsin-AuNPs
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