User:Eleni N. Kalivas/Notebook/CHEM-571/2013/10/30: Difference between revisions
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<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
== | ==Objective== | ||
* | The objective for today is to synthesize new Myoglobin-AuNP solutions with different ratios in order to determine the optimal concentration of Au:protein for the formation of fibers, and to also synthesize Lysozyme-AuNP solutions | ||
==Protocol== | |||
*5mL of each solution was prepared. | |||
<br> | |||
Preparation of Myoglobin-AuNP solutions | |||
<br> | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Ratio Myoglobin''' | |||
| align="center" style="background:#f0f0f0;"|'''Amount of gold added(mL)''' | |||
| align="center" style="background:#f0f0f0;"|'''Concentration Myoglobin(M)''' | |||
| align="center" style="background:#f0f0f0;"|'''Volume Myoglobin(mL)''' | |||
| align="center" style="background:#f0f0f0;"|'''Volume Water Myoglobin(mL)''' | |||
|- | |||
| 5||0.494264558||0.00005||2||2.505735442 | |||
|- | |||
| 10||0.494264558||0.000025||1||3.505735442 | |||
|- | |||
| 15||0.494264558||1.66667E-05||0.666666667||3.839068775 | |||
|- | |||
| 20||0.494264558||0.0000125||0.5||4.005735442 | |||
|- | |||
| 25||0.494264558||0.00001||0.4||4.105735442 | |||
|- | |||
| 30||0.494264558||8.33333E-06||0.333333333||4.172402108 | |||
|- | |||
| 35||0.494264558||7.14286E-06||0.285714286||4.220021156 | |||
|- | |||
| 40||0.494264558||0.00000625||0.25||4.255735442 | |||
|- | |||
| 45||0.494264558||5.55556E-06||0.222222222||4.28351322 | |||
|- | |||
| 50||0.494264558||0.000005||0.2||4.305735442 | |||
|} | |||
<br> | |||
Preparation of all Lysozyme-AuNP solutions | |||
<br> | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Ratio Lysozyme''' | |||
| align="center" style="background:#f0f0f0;"|'''Amount gold added (mL)''' | |||
| align="center" style="background:#f0f0f0;"|'''Concentration Lysozyme(M)''' | |||
| align="center" style="background:#f0f0f0;"|'''Volume Lysozyme (mL)''' | |||
| align="center" style="background:#f0f0f0;"|'''Volume Water Lysozyme (mL)''' | |||
|- | |||
| 30||0.494264558||8.33333E-06||1.611148649||2.894586793 | |||
|- | |||
| 50||0.494264558||0.000005||0.966689189||3.539046253 | |||
|- | |||
| 70||0.494264558||3.57143E-06||0.690492278||3.815243164 | |||
|- | |||
| 90||0.494264558||2.77778E-06||0.53704955||3.968685892 | |||
|- | |||
| 110||0.494264558||2.27273E-06||0.439404177||4.066331265 | |||
|- | |||
| 130||0.494264558||1.92308E-06||0.371803534||4.133931907 | |||
|- | |||
| 150||0.494264558||1.66667E-06||0.32222973||4.183505712 | |||
|- | |||
| 170||0.494264558||1.47059E-06||0.28432035||4.221415092 | |||
|- | |||
| 190||0.494264558||1.31579E-06||0.254391892||4.25134355 | |||
|- | |||
| 210||0.494264558||1.19048E-06||0.230164093||4.275571349 | |||
|} | |||
<br> | |||
Stock Solution concentrations | |||
<br> | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Concentration of Myoglobin (M)''' | |||
| align="center" style="background:#f0f0f0;"|'''Concentration Gold(M)''' | |||
| align="center" style="background:#f0f0f0;"|'''Concentration Lysozyme (M)''' | |||
|- | |||
| 0.000125||0.00252901||2.58615E-05 | |||
|} | |||
*All stock solutions were prepared with deionized H<sub>2</sub>O | |||
*The gold stock solution was prepared using Gold Chloride (II) trihydrate | |||
<br> | |||
Preparation of Lysozyme Stock solution | |||
<br> | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Molecular Weight(g/mol)''' | |||
| align="center" style="background:#f0f0f0;"|'''14307''' | |||
|- | |||
| mass lysozyme(g)||0.0037 | |||
|- | |||
| volume solution(mL)||10 | |||
|- | |||
| Concentration Lysozyme (M)||2.58615E-05 | |||
|} | |||
<br> | |||
Preparation of Myoglobin Stock solution | |||
<br> | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Molecular weight (g/mol)''' | |||
| align="center" style="background:#f0f0f0;"|'''17600''' | |||
|- | |||
| mass myoglobin (g)||0.022 | |||
|- | |||
| volume solution (mL)||0.01 | |||
|- | |||
| Concentration (M)||0.000125 | |||
|} | |||
<br> | |||
==Data== | |||
We will run UV-vis on the prepared samples next at some point next week. | |||
Revision as of 11:58, 5 November 2013
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ObjectiveThe objective for today is to synthesize new Myoglobin-AuNP solutions with different ratios in order to determine the optimal concentration of Au:protein for the formation of fibers, and to also synthesize Lysozyme-AuNP solutions Protocol
DataWe will run UV-vis on the prepared samples next at some point next week.
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