User:Eleni N. Kalivas/Notebook/CHEM-571/2013/10/30: Difference between revisions

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==Objective==
==Objective==
The objective for today is to run AA on the HRP-AuNPs and pepsin-AuNPs from last week and also on the Myoglobin-AuNPs and HRP-AuNPs from this week.
The objective for today is to synthesize new Myoglobin-AuNP solutions with different ratios in order to determine the optimal concentration of Au:protein for the formation of fibers, and to also synthesize Lysozyme-AuNP solutions


==Protocol==
==Protocol==

Revision as of 12:09, 30 October 2013

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Objective

The objective for today is to synthesize new Myoglobin-AuNP solutions with different ratios in order to determine the optimal concentration of Au:protein for the formation of fibers, and to also synthesize Lysozyme-AuNP solutions

Protocol

Atomic Absorption

    1. centrifuge (4mL of your) samples (use the large centrifuge upstairs) 30min at 4000rpm.
    2. make standard solutions in the same manner as the last time we performed AA. But, this time make concentrations that go up to 50ug/mL
    3. Take measurements of your standards and samples
    4. Make a graph showing concentration of Au vs Au:protein ratio

Data


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