User:Eleni N. Kalivas/Notebook/CHEM-571/2013/10/30

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(Autocreate 2013/10/30 Entry for User:Eleni_N._Kalivas/Notebook/CHEM-571)
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==Entry title==
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==Objective==
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The objective for today is to run AA on the HRP-AuNPs and pepsin-AuNPs from last week and also on the Myoglobin-AuNPs and HRP-AuNPs from this week.  
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==Protocol==
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Atomic Absorption
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## centrifuge (4mL of your) samples (use the large centrifuge upstairs) 30min at 4000rpm.  
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## make standard solutions in the same manner as the last time we performed AA. But, this time make concentrations that go up to 50ug/mL
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## Take measurements of your standards and samples
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## Make a graph showing concentration of Au vs Au:protein ratio
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==Data==

Revision as of 17:31, 29 October 2013

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Objective

The objective for today is to run AA on the HRP-AuNPs and pepsin-AuNPs from last week and also on the Myoglobin-AuNPs and HRP-AuNPs from this week.

Protocol

Atomic Absorption

    1. centrifuge (4mL of your) samples (use the large centrifuge upstairs) 30min at 4000rpm.
    2. make standard solutions in the same manner as the last time we performed AA. But, this time make concentrations that go up to 50ug/mL
    3. Take measurements of your standards and samples
    4. Make a graph showing concentration of Au vs Au:protein ratio

Data


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