User:Eleni N. Kalivas/Notebook/CHEM-571/2013/10/30

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(Objective)
(Protocol)
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==Protocol==
==Protocol==
-
Atomic Absorption
+
*5mL of each solution was prepared.
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## centrifuge (4mL of your) samples (use the large centrifuge upstairs) 30min at 4000rpm.
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<br>
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## make standard solutions in the same manner as the last time we performed AA. But, this time make concentrations that go up to 50ug/mL
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Preparation of Myoglobin-AuNP solutions
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## Take measurements of your standards and samples
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<br>
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## Make a graph showing concentration of Au vs Au:protein ratio
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Ratio Myoglobin'''
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| align="center" style="background:#f0f0f0;"|'''Amount of gold added(mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''Concentration Myoglobin(M)'''
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| align="center" style="background:#f0f0f0;"|'''Volume Myoglobin(mL)'''
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| align="center" style="background:#f0f0f0;"|'''Volume Water Myoglobin(mL)'''
 +
|-
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| 5||0.494264558||0.00005||2||2.505735442
 +
|-
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| 10||0.494264558||0.000025||1||3.505735442
 +
|-
 +
| 15||0.494264558||1.66667E-05||0.666666667||3.839068775
 +
|-
 +
| 20||0.494264558||0.0000125||0.5||4.005735442
 +
|-
 +
| 25||0.494264558||0.00001||0.4||4.105735442
 +
|-
 +
| 30||0.494264558||8.33333E-06||0.333333333||4.172402108
 +
|-
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| 35||0.494264558||7.14286E-06||0.285714286||4.220021156
 +
|-
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| 40||0.494264558||0.00000625||0.25||4.255735442
 +
|-
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| 45||0.494264558||5.55556E-06||0.222222222||4.28351322
 +
|-
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| 50||0.494264558||0.000005||0.2||4.305735442
 +
|}
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<br>
 +
Preparation of all Lysozyme-AuNP solutions
 +
<br>
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''Ratio Lysozyme'''
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| align="center" style="background:#f0f0f0;"|'''Amount gold added (mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''Concentration Lysozyme(M)'''
 +
| align="center" style="background:#f0f0f0;"|'''Volume Lysozyme (mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''Volume Water Lysozyme (mL)'''
 +
|-
 +
| 30||0.494264558||8.33333E-06||1.611148649||2.894586793
 +
|-
 +
| 50||0.494264558||0.000005||0.966689189||3.539046253
 +
|-
 +
| 70||0.494264558||3.57143E-06||0.690492278||3.815243164
 +
|-
 +
| 90||0.494264558||2.77778E-06||0.53704955||3.968685892
 +
|-
 +
| 110||0.494264558||2.27273E-06||0.439404177||4.066331265
 +
|-
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| 130||0.494264558||1.92308E-06||0.371803534||4.133931907
 +
|-
 +
| 150||0.494264558||1.66667E-06||0.32222973||4.183505712
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|-
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| 170||0.494264558||1.47059E-06||0.28432035||4.221415092
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|-
 +
| 190||0.494264558||1.31579E-06||0.254391892||4.25134355
 +
|-
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| 210||0.494264558||1.19048E-06||0.230164093||4.275571349
 +
|}
 +
<br>
 +
Stock Solution concentrations
 +
<br>
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''Concentration of Myoglobin (M)'''
 +
| align="center" style="background:#f0f0f0;"|'''Concentration Gold(M)'''
 +
| align="center" style="background:#f0f0f0;"|'''Concentration Lysozyme (M)'''
 +
|-
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| 0.000125||0.00252901||2.58615E-05
 +
|}
 +
*All stock solutions were prepared with deionized H<sub>2</sub>O
 +
*The gold stock solution was prepared using Gold Chloride (II) trihydrate
 +
<br>
 +
Preparation of Lysozyme Stock solution
 +
<br>
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''Molecular Weight(g/mol)'''
 +
| align="center" style="background:#f0f0f0;"|'''14307'''
 +
|-
 +
| mass lysozyme(g)||0.0037
 +
|-
 +
| volume solution(mL)||10
 +
|-
 +
| Concentration Lysozyme (M)||2.58615E-05
 +
|}
 +
<br>
 +
Preparation of Myoglobin Stock solution
 +
<br>
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''Molecular weight (g/mol)'''
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| align="center" style="background:#f0f0f0;"|'''17600'''
 +
|-
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| mass myoglobin (g)||0.022
 +
|-
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| volume solution (mL)||0.01
 +
|-
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| Concentration (M)||0.000125
 +
|}
 +
<br>
==Data==
==Data==

Revision as of 15:10, 30 October 2013

Project name Main project page
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Objective

The objective for today is to synthesize new Myoglobin-AuNP solutions with different ratios in order to determine the optimal concentration of Au:protein for the formation of fibers, and to also synthesize Lysozyme-AuNP solutions

Protocol

  • 5mL of each solution was prepared.


Preparation of Myoglobin-AuNP solutions

Ratio Myoglobin Amount of gold added(mL) Concentration Myoglobin(M) Volume Myoglobin(mL) Volume Water Myoglobin(mL)
50.4942645580.0000522.505735442
100.4942645580.00002513.505735442
150.4942645581.66667E-050.6666666673.839068775
200.4942645580.00001250.54.005735442
250.4942645580.000010.44.105735442
300.4942645588.33333E-060.3333333334.172402108
350.4942645587.14286E-060.2857142864.220021156
400.4942645580.000006250.254.255735442
450.4942645585.55556E-060.2222222224.28351322
500.4942645580.0000050.24.305735442


Preparation of all Lysozyme-AuNP solutions

Ratio Lysozyme Amount gold added (mL) Concentration Lysozyme(M) Volume Lysozyme (mL) Volume Water Lysozyme (mL)
300.4942645588.33333E-061.6111486492.894586793
500.4942645580.0000050.9666891893.539046253
700.4942645583.57143E-060.6904922783.815243164
900.4942645582.77778E-060.537049553.968685892
1100.4942645582.27273E-060.4394041774.066331265
1300.4942645581.92308E-060.3718035344.133931907
1500.4942645581.66667E-060.322229734.183505712
1700.4942645581.47059E-060.284320354.221415092
1900.4942645581.31579E-060.2543918924.25134355
2100.4942645581.19048E-060.2301640934.275571349


Stock Solution concentrations

Concentration of Myoglobin (M) Concentration Gold(M) Concentration Lysozyme (M)
0.0001250.002529012.58615E-05
  • All stock solutions were prepared with deionized H2O
  • The gold stock solution was prepared using Gold Chloride (II) trihydrate


Preparation of Lysozyme Stock solution

Molecular Weight(g/mol) 14307
mass lysozyme(g)0.0037
volume solution(mL)10
Concentration Lysozyme (M)2.58615E-05


Preparation of Myoglobin Stock solution

Molecular weight (g/mol) 17600
mass myoglobin (g)0.022
volume solution (mL)0.01
Concentration (M)0.000125


Data


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