User:Eleni N. Kalivas/Notebook/CHEM-571/2013/11/05: Difference between revisions

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==Entry title==
==Objective==
* Insert content here...
will be doing AA on their samples from last week (HRP-AuNPs and pepsin-AuNPs) and this week (Myoglobin-AuNPs and HRP-AuNPs).


==Protocol==
Atomic Absorption
## centrifuge (4mL of your) samples (use the large centrifuge upstairs) 30min at 4000rpm.
## make standard solutions in the same manner as the last time we performed AA. But, this time make concentrations that go up to 50ug/mL
## Take measurements of your standards and samples
## Make a graph showing concentration of Au vs Au:protein ratio
Standard Au Solution preparation
(from 1000μg/mL AA Au gold standard solution)
<br>
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Concentration(μg/mL)'''
| align="center" style="background:#f0f0f0;"|'''Volume of stock Au solution(mL)'''
| align="center" style="background:#f0f0f0;"|'''Volume Deionized water added(mL)'''
|-
| 10||0.1||9.9
|-
| 20||0.2||9.8
|-
| 30||0.3||9.7
|-
| 40||0.4||9.6
|-
| 50||0.5||9.5
|}
==Data==
* Atomic Absorbance Gold Standard Calibration Curve
[[Image:CalibrationcurveAAmyohemozem.png|750px]]
*Hemoglobin Absorbance Curve
[[Image:Concentration vs Absorbance Hemoglobin.jpg|750px]]
*Myoglobin Absorbance Curve
[[Image:Concentration vs Absorbance Myoglobin.jpg|750px]]
==Additional Observations==
Lysozyme solutions all produced fibers and appear colorless except for the 30:1 ratio, which contained light pigmentation.
All myoglobin solutions produced fibers and appear colorless.


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Revision as of 15:54, 20 November 2013

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Objective

will be doing AA on their samples from last week (HRP-AuNPs and pepsin-AuNPs) and this week (Myoglobin-AuNPs and HRP-AuNPs).

Protocol

Atomic Absorption

    1. centrifuge (4mL of your) samples (use the large centrifuge upstairs) 30min at 4000rpm.
    2. make standard solutions in the same manner as the last time we performed AA. But, this time make concentrations that go up to 50ug/mL
    3. Take measurements of your standards and samples
    4. Make a graph showing concentration of Au vs Au:protein ratio

Standard Au Solution preparation (from 1000μg/mL AA Au gold standard solution)

Concentration(μg/mL) Volume of stock Au solution(mL) Volume Deionized water added(mL)
10 0.1 9.9
20 0.2 9.8
30 0.3 9.7
40 0.4 9.6
50 0.5 9.5

Data

  • Atomic Absorbance Gold Standard Calibration Curve

  • Hemoglobin Absorbance Curve

  • Myoglobin Absorbance Curve

Additional Observations

Lysozyme solutions all produced fibers and appear colorless except for the 30:1 ratio, which contained light pigmentation. All myoglobin solutions produced fibers and appear colorless.


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