User:Eleni N. Kalivas/Notebook/CHEM-571/2013/11/06

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==Entry title==
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==Objective==
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Our objective for today was to run UV-vis on the Lysozyme-AuNPs and Myoglobin-AuNPs samples that were synthesized last week. Today we will also be synthesizing new lysozyme-AuNPs and also new Hemoglobin-AuNPs solutions
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==Protocol==
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*The samples that were being run on the UV-vis were first centrifuged for 30 minutes before each spectra was taken.
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*New AuNP solutions
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#Lysozyme-AuNPs (protein:Au ratio):
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##5:1
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##10:1
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##15:1
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##20:1
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##25:1
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##30:1
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##35:1
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##40:1
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##45:1
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##50:1
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#Hemoglobin-AuNPs (protein:Au ratio)
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##30:1
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##50:1
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##70:1
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##90:1
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##110:1
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##130:1
 +
##150:1
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##170:1
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##190:1
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##210:1
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Table of Lysozyme-AuNP preparation
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Table of Hemoglobin-AuNP preparation
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==Data==
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Absorbance Spectra of Lysozyme-AuNPs
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[[Image:Corrected Absorbance Spectra of Lysozyme-AuNP solutions.png|750 px]]
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Absorbance of Myoglobin-AuNPs
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[[Image:Corrected Absorbance spectra of Myoglobin Au-NP solution .png|750 px]]

Revision as of 12:30, 7 November 2013

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Objective

Our objective for today was to run UV-vis on the Lysozyme-AuNPs and Myoglobin-AuNPs samples that were synthesized last week. Today we will also be synthesizing new lysozyme-AuNPs and also new Hemoglobin-AuNPs solutions

Protocol

  • The samples that were being run on the UV-vis were first centrifuged for 30 minutes before each spectra was taken.
  • New AuNP solutions
  1. Lysozyme-AuNPs (protein:Au ratio):
    1. 5:1
    2. 10:1
    3. 15:1
    4. 20:1
    5. 25:1
    6. 30:1
    7. 35:1
    8. 40:1
    9. 45:1
    10. 50:1
  2. Hemoglobin-AuNPs (protein:Au ratio)
    1. 30:1
    2. 50:1
    3. 70:1
    4. 90:1
    5. 110:1
    6. 130:1
    7. 150:1
    8. 170:1
    9. 190:1
    10. 210:1

Table of Lysozyme-AuNP preparation

Table of Hemoglobin-AuNP preparation

Data

Absorbance Spectra of Lysozyme-AuNPs

Absorbance of Myoglobin-AuNPs



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