User:Eleni N. Kalivas/Notebook/CHEM-571/2013/11/06

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(Protocol)
(Protocol)
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==Protocol==
==Protocol==
*The samples that were being run on the UV-vis were first centrifuged for 30 minutes before each spectra was taken.
*The samples that were being run on the UV-vis were first centrifuged for 30 minutes before each spectra was taken.
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*New AuNP solutions
+
*Protein:AuNP solutions, details of how the solutions were prepared can be found [[User:Moira M. Esson/Notebook/CHEM-571/2013/11/06|here]].
#Lysozyme-AuNPs (protein:Au ratio):
#Lysozyme-AuNPs (protein:Au ratio):
##5:1
##5:1

Revision as of 19:27, 20 November 2013

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Objective

Our objective for today was to run UV-vis on the Lysozyme-AuNPs and Myoglobin-AuNPs samples that were synthesized last week. Today we will also be synthesizing new lysozyme-AuNPs and also new Hemoglobin-AuNPs solutions

Protocol

  • The samples that were being run on the UV-vis were first centrifuged for 30 minutes before each spectra was taken.
  • Protein:AuNP solutions, details of how the solutions were prepared can be found here.
  1. Lysozyme-AuNPs (protein:Au ratio):
    1. 5:1
    2. 10:1
    3. 15:1
    4. 20:1
    5. 25:1
    6. 30:1
    7. 35:1
    8. 40:1
    9. 45:1
    10. 50:1
  2. Hemoglobin-AuNPs (protein:Au ratio)
    1. 30:1
    2. 50:1
    3. 70:1
    4. 90:1
    5. 110:1
    6. 130:1
    7. 150:1
    8. 170:1
    9. 190:1
    10. 210:1

Data

Absorbance Spectra of Lysozyme-AuNPs

Absorbance of Myoglobin-AuNPs



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