User:Eleni N. Kalivas/Notebook/CHEM-571/2013/11/13: Difference between revisions

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==Protocol==  
==Protocol==  
Approximately 4mL of each sample was centrifuged for 30 minutes. Five gold standards were prepared with the following concentrations:
Approximately 4mL of each sample was centrifuged for 30 minutes. Five gold standards were prepared with the following concentrations:
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Concentration(μg/mL)'''
| align="center" style="background:#f0f0f0;"|'''Volume of stock Au solution(mL)'''
| align="center" style="background:#f0f0f0;"|'''Volume Deionized water added(mL)'''
|-
| 10||0.1||9.9
|-
| 20||0.2||9.8
|-
| 30||0.3||9.7
|-
| 40||0.4||9.6
|-
| 50||0.5||9.5
|}


==Data==
==Data==
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Concentration vs. Absorbance Hemoglobin-AuNP solutions
Concentration vs. Absorbance Hemoglobin-AuNP solutions
[[Image:Concentration vs. Absorbance AuNP-Hemoglobin solutions zem.png|750px]]
[[Image:Concentration vs. Absorbance AuNP-Hemoglobin solutions zem.png|750px]]
==Additional Observations==
*The AuNP-protein solutions prepared on 10/30/2013 will be analyzed during the next period. These samples contained a smaller volume (3.5mL) than the other volumes being centrifuged and as such will be analyzed during the next lab period after being centrifuged.


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Revision as of 17:11, 20 November 2013

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Objective

The objective for today's lab was to analyze the solutions prepared on 11/06/13 using Atomic Absorbance

Protocol

Approximately 4mL of each sample was centrifuged for 30 minutes. Five gold standards were prepared with the following concentrations:

Concentration(μg/mL) Volume of stock Au solution(mL) Volume Deionized water added(mL)
10 0.1 9.9
20 0.2 9.8
30 0.3 9.7
40 0.4 9.6
50 0.5 9.5

Data

Calibration curve of Gold Standards

Atomic Absorption spectra of Lysozyme

Atomic ABsorbance Spectra of Hemoglobin

Concentration vs. Absorbance Lysozyme-AuNP solutions

Concentration vs. Absorbance Hemoglobin-AuNP solutions

Additional Observations

  • The AuNP-protein solutions prepared on 10/30/2013 will be analyzed during the next period. These samples contained a smaller volume (3.5mL) than the other volumes being centrifuged and as such will be analyzed during the next lab period after being centrifuged.


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