User:Eleni N. Kalivas/Notebook/CHEM-571/2014/01/28: Difference between revisions

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==Objective==
==Objective==
The objective for today was to make all stock solutions necessary for the BSA:L-cysteine Nanocrystals
The objective for today was to make all stock solutions necessary for the BSA:L-cysteine Nanocrystals
==Materials==
{| {{table}}
| align="center" style="background:#f0f0f0;"|''''''
|-
| Name
|-
| Formula
|-
| FW (g/mol)
|-
| Zinc Nitrate, 6-Hydrate Crystal
|-
| Zn(NO3)2 * 6H2O
|-
| 297.470
|-
| Manganese (II) acetate tetrahydrate (good for 2 weeks)
|-
| Mn(CH3COO)2 * 4H2O
|-
|  245.08
|-
| Ammonium Acetate
|-
| NH4(CH3COO)
|-
| 77.08
|-
| Sodium Sulfide nonahydrate(fresh stock)
|-
| Na2S * 9 H2O
|-
| 240.18
|-
| L-Cysteine (2 weeks)
|-
| HSCH2CH(NH2)CO2H
|-
| 121.16
|-
| BSA (fresh stock)
|-
|
|-
|
|-
| 66kDa
|-
| Ammonium Hydroxide
|-
| NH4OH
|-
| 14.8M (6.76uL in 10 mL)
|-
|
|}


==Preparation of Stocks==
==Preparation of Stocks==

Revision as of 13:25, 28 January 2014

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Objective

The objective for today was to make all stock solutions necessary for the BSA:L-cysteine Nanocrystals

Materials

'
Name
Formula
FW (g/mol)
Zinc Nitrate, 6-Hydrate Crystal
Zn(NO3)2 * 6H2O
297.470
Manganese (II) acetate tetrahydrate (good for 2 weeks)
Mn(CH3COO)2 * 4H2O
245.08
Ammonium Acetate
NH4(CH3COO)
77.08
Sodium Sulfide nonahydrate(fresh stock)
Na2S * 9 H2O
240.18
L-Cysteine (2 weeks)
HSCH2CH(NH2)CO2H
121.16
BSA (fresh stock)
66kDa
Ammonium Hydroxide
NH4OH
14.8M (6.76uL in 10 mL)

Preparation of Stocks

  1. 5mM Zinc Nitrate
  2. 5.55mM Manganese Acetate
  3. 41.65 mM Ammonium Acetate


  • 0.0066g in 10mL BSA will be made tomorrow