User:Eleni N. Kalivas/Notebook/CHEM-571/2014/01/29: Difference between revisions

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| align="center" style="background:#f0f0f0;"|'''2.00E-10'''
| align="center" style="background:#f0f0f0;"|'''2.00E-10'''
| align="center" style="background:#f0f0f0;"|'''0'''
| align="center" style="background:#f0f0f0;"|'''0'''
|-
| percentage of protein||100||90||80||70||60||50||40||30||20||10||0
|-
| percentage of ligand||0||10||20||30||40||50||60||70||80||90||100
|-
| mol of ligand||0||2.00E-10||4.00E-10||6.00E-10||8.00E-10||1.00E-09||1.20E-09||1.40E-09||1.60E-09||1.80E-09||2.00E-09
|}
{| {{table}}
| mol of protein||2.00E-09||1.80E-09||1.60E-09||1.40E-09||1.20E-09||1.00E-09||8.00E-10||6.00E-10||4.00E-10||2.00E-10||0
|-
|-
| percentage of protein||100||90||80||70||60||50||40||30||20||10||0
| percentage of protein||100||90||80||70||60||50||40||30||20||10||0

Revision as of 13:24, 28 January 2014

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objective

The objective for today is to begin the synthesis of BSA:L-Cysteine Nanocrystals

Preparation of stock solutions

  1. BSA
  2. L-Cysteine
  3. Sodium Hydroxide
  4. Sodium Sulfide

Protocol BSA:L-cysteine Nanocrystals

  1. In test tube mix 180 uL of 5mM Zinc Nitrate and 20 uL of 5mM Manganese Acetate
  2. Add 200 uL of 40 mM Ammonium Acetate
  3. Add 200 uL of Protein:ligand in various ratios from 0% to 100% protein
  4. Adjust pH to 8.2 using roughly 250 uL of 10mM Ammonium Hydroxide, and bring volume of water to 2.3 mL
  5. Incubate at room temp for 1 hour
  6. Add 200 uL of 5mM Sodium Sulfide drop wise while vortexing
  7. Incubate for five days at 37 degrees Celsius

Table of BSA:L-Cysteine ratios

mol of protein 2.00E-09 1.80E-09 1.60E-09 1.40E-09 1.20E-09 1.00E-09 8.00E-10 6.00E-10 4.00E-10 2.00E-10 0
percentage of protein 100 90 80 70 60 50 40 30 20 10 0
percentage of ligand 0 10 20 30 40 50 60 70 80 90 100
mol of ligand 0 2.00E-10 4.00E-10 6.00E-10 8.00E-10 1.00E-09 1.20E-09 1.40E-09 1.60E-09 1.80E-09 2.00E-09
mol of protein 2.00E-09 1.80E-09 1.60E-09 1.40E-09 1.20E-09 1.00E-09 8.00E-10 6.00E-10 4.00E-10 2.00E-10 0
percentage of protein 100 90 80 70 60 50 40 30 20 10 0
percentage of ligand 0 10 20 30 40 50 60 70 80 90 100
mol of ligand 0 2.00E-10 4.00E-10 6.00E-10 8.00E-10 1.00E-09 1.20E-09 1.40E-09 1.60E-09 1.80E-09 2.00E-09



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