User:Eleni N. Kalivas/Notebook/CHEM-571/2014/02/12: Difference between revisions

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==BSA-GSH NC Protocol==  
==BSA-GSH NC Table of volumes==
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''% BSA'''
| align="center" style="background:#f0f0f0;"|'''5 mM Zinc Nitrate (uL)'''
| align="center" style="background:#f0f0f0;"|'''5mM Manganese Acetate (uL)'''
| align="center" style="background:#f0f0f0;"|'''41.65 mM Ammonium Acetate (uL)'''
| align="center" style="background:#f0f0f0;"|'''10uM BSA* (uL)'''
| align="center" style="background:#f0f0f0;"|'''10uM GSH (uL)'''
| align="center" style="background:#f0f0f0;"|'''10mM Ammonium Hydroxide (uL)'''
| align="center" style="background:#f0f0f0;"|'''5 mM Sodium Sulfide* (uL)'''
| align="center" style="background:#f0f0f0;"|'''Total Volume (mL)'''
|-
| 70||540||60||600||420||180||3000||600||5.4
|-
| 75||540||60||600||450||150||3000||600||5.4
|-
| 80||540||60||600||480||120||3000||600||5.4
|-
| 85||540||60||600||510||90||3000||600||5.4
|-
| 90||540||60||600||540||60||3000||600||5.4
|-
| 95||540||60||600||570||30||3000||600||5.4
|-
| 100||540||60||600||600||0||3000||600||5.4
|}


==BSA-1-thioglycerol NC Protocol==
==BSA-1-thioglycerol NC Protocol==

Revision as of 11:09, 11 February 2014

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Protocol

BSA-L-cysteine NC table of volumes

% BSA 5 mM Zinc Nitrate (uL) 5mM Manganese Acetate (uL) 41.65 mM Ammonium Acetate (uL) 11uM BSA* (uL) 10uM L-Cysteine (uL) 10mM Ammonium Hydroxide (uL) 5 mM Sodium Sulfide* (uL) Total Volume (mL)
80 540 60 600 480 120 3000 600 5.4
85 540 60 600 510 90 3000 600 5.4
90 540 60 600 540 60 3000 600 5.4
95 540 60 600 570 30 3000 600 5.4
100 540 60 600 600 0 3000 600 5.4

BSA-GSH NC Table of volumes

% BSA 5 mM Zinc Nitrate (uL) 5mM Manganese Acetate (uL) 41.65 mM Ammonium Acetate (uL) 10uM BSA* (uL) 10uM GSH (uL) 10mM Ammonium Hydroxide (uL) 5 mM Sodium Sulfide* (uL) Total Volume (mL)
70 540 60 600 420 180 3000 600 5.4
75 540 60 600 450 150 3000 600 5.4
80 540 60 600 480 120 3000 600 5.4
85 540 60 600 510 90 3000 600 5.4
90 540 60 600 540 60 3000 600 5.4
95 540 60 600 570 30 3000 600 5.4
100 540 60 600 600 0 3000 600 5.4

BSA-1-thioglycerol NC Protocol

  • This is the first round of synthesis, therefore a ratio at which the NC's are stable in solution has not been identified and we will conduct standard ratios.



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