User:Eleni N. Kalivas/Notebook/CHEM-571/2014/02/12: Difference between revisions
From OpenWetWare
Line 6: | Line 6: | ||
| colspan="2"| | | colspan="2"| | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
=Protocol= | =General Protocol= | ||
# In test tube mix 180 uL of 5mM Zinc Nitrate and 20 uL of 5mM Manganese Acetate | |||
# Add 200 uL of 40 mM Ammonium Acetate | |||
# Add 200 uL of Protein:ligand in various ratios from 0% to 100% protein | |||
# Adjust pH to 8.2 using roughly 250 uL of 10mM Ammonium Hydroxide | |||
# Incubate at room temp for 1 hour | |||
# Add 200 uL of 5mM Sodium Sulfide drop wise, then cover the test tubes | |||
#A VWR Analog Vortex Mixer was used to vortex each test tube for 30 seconds | |||
# Incubate for five-six days at 37 degrees Celsius | |||
==BSA-L-cysteine NC table of volumes== | ==BSA-L-cysteine NC table of volumes== |
Revision as of 11:11, 11 February 2014
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
General Protocol
BSA-L-cysteine NC table of volumes
BSA-GSH NC Table of volumes
BSA-1-thioglycerol table of volumes
|