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==Data== | ==Data== | ||
* | |||
*Double Concentration Tables of volumes | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''% BSA''' | |||
| align="center" style="background:#f0f0f0;"|'''10 mM Zinc Nitrate (uL)''' | |||
| align="center" style="background:#f0f0f0;"|'''10mM Manganese Acetate (uL)''' | |||
| align="center" style="background:#f0f0f0;"|'''80 mM Ammonium Acetate (uL)''' | |||
| align="center" style="background:#f0f0f0;"|'''20uM BSA* (uL)''' | |||
| align="center" style="background:#f0f0f0;"|'''20uM L-Cysteine (uL)''' | |||
| align="center" style="background:#f0f0f0;"|'''20mM Ammonium Hydroxide (uL)''' | |||
| align="center" style="background:#f0f0f0;"|'''10 mM Sodium Sulfide* (uL)''' | |||
| align="center" style="background:#f0f0f0;"|'''Total Volume (mL)''' | |||
|- | |||
| 80||540||60||600||480||120||3000||600||5.4 | |||
|- | |||
| 85||540||60||600||510||90||3000||600||5.4 | |||
|- | |||
| 90||540||60||600||540||60||3000||600||5.4 | |||
|- | |||
| 95||540||60||600||570||30||3000||600||5.4 | |||
|- | |||
| 100||540||60||600||600||0||3000||600||5.4 | |||
|} | |||
*Half Concentration | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''% BSA''' | |||
| align="center" style="background:#f0f0f0;"|'''2.5 mM Zinc Nitrate (uL)''' | |||
| align="center" style="background:#f0f0f0;"|'''2.5 mM Manganese Acetate (uL)''' | |||
| align="center" style="background:#f0f0f0;"|'''20 mM Ammonium Acetate (uL)''' | |||
| align="center" style="background:#f0f0f0;"|'''5 uM BSA* (uL)''' | |||
| align="center" style="background:#f0f0f0;"|'''5 uM L-Cysteine (uL)''' | |||
| align="center" style="background:#f0f0f0;"|'''5 mM Ammonium Hydroxide (uL)''' | |||
| align="center" style="background:#f0f0f0;"|'''2.5 mM Sodium Sulfide* (uL)''' | |||
| align="center" style="background:#f0f0f0;"|'''Total Volume (mL)''' | |||
|- | |||
| 80||540||60||600||480||120||3000||600||5.4 | |||
|- | |||
| 85||540||60||600||510||90||3000||600||5.4 | |||
|- | |||
| 90||540||60||600||540||60||3000||600||5.4 | |||
|- | |||
| 95||540||60||600||570||30||3000||600||5.4 | |||
|- | |||
| 100||540||60||600||600||0||3000||600||5.4 | |||
|} | |||
Large Volumen Synthesis | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''% BSA''' | |||
| align="center" style="background:#f0f0f0;"|'''2.5 mM Zinc Nitrate (uL)''' | |||
| align="center" style="background:#f0f0f0;"|'''2.5 mM Manganese Acetate (uL)''' | |||
| align="center" style="background:#f0f0f0;"|'''20 mM Ammonium Acetate (uL)''' | |||
| align="center" style="background:#f0f0f0;"|'''5 uM BSA* (uL)''' | |||
| align="center" style="background:#f0f0f0;"|'''5 uM L-Cysteine (uL)''' | |||
| align="center" style="background:#f0f0f0;"|'''5 mM Ammonium Hydroxide (uL)''' | |||
| align="center" style="background:#f0f0f0;"|'''2.5 mM Sodium Sulfide* (uL)''' | |||
| align="center" style="background:#f0f0f0;"|'''Total Volume (mL)''' | |||
|- | |||
| 80||2160||240||2400||1920||480||12000||2400||21.6 | |||
|} | |||
==Data== | |||
Absorbance 80% BSA-L-cysteine half concentration | |||
[[Image:UV-Vis.BSA-C80%halfconcentration.png|750px]] | |||
Fluorescence 80% BSA-L-cysteine half concentration | |||
[[Image:Fluorescence.BSA-C805halfconcentration.png|750px]] | |||
==Notes== | ==Notes== |
Revision as of 10:35, 15 April 2014
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Objective
Description
Data
Large Volumen Synthesis
DataAbsorbance 80% BSA-L-cysteine half concentration Fluorescence 80% BSA-L-cysteine half concentration NotesThis area is for any observations or conclusions that you would like to note.
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