User:Eleni N. Kalivas/Notebook/CHEM-571/2014/03/05: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(2 intermediate revisions by the same user not shown)
Line 7: Line 7:
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==Objective==
==Objective==
*To start the new synthesis method
*Continue Data Analysis
*Precipitate NC QDs synthesized yesterday


==Protocol==  
==Data==
This protocol was taken from [[User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2014/02/03|Matt Harting's]] notebook
Absorbance of 78% BSA by mass (L-cysteine)
[[Image:78percbymassBSA-Cys.png|750px]]
 
Fluorescence of 78% BSA by mass (L-cysteine)
[[Image:78%bymassBSA-Cys.png|750px]]


# To a 3-necked flask is added
## 25mL of 20 mM L-cysteine (roughly 22% L-Cysteine)
## 25mL .1459 mM BSA (roughly 78% by mass)
## 5mL of 100mM ZnSO<sub>4</sub> (0.5 mmol)
## 5.437mL of 10.3mM MnCl<sub>2</sub> (0.056 mmol)
## The pH of this mixture is adjusted to 11 with ~1M NaOH (should be on the order of 60uL)
# This mixture is stirred under Ar (I'll use N<sub>2</sub>) for 30 minutes
# 5.505mL of 101mM Na<sub>2</sub>S is quickly added.
# The solution is stirred for 20 minutes
# The solution is then aged at 50C under air for 2 hours.




Revision as of 12:12, 15 April 2014

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Objective

  • Continue Data Analysis
  • Precipitate NC QDs synthesized yesterday

Data

Absorbance of 78% BSA by mass (L-cysteine)

Fluorescence of 78% BSA by mass (L-cysteine)



Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Eleni_N._Kalivas/Notebook/CHEM-571/2014/03/05&oldid=787794"