User:Eleni N. Kalivas/Notebook/CHEM-571/2014/03/05

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(Protocol)
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*To start the new synthesis method
*To start the new synthesis method
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==Protocol==
 
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This protocol was taken from [[User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2014/02/03|Matt Harting's]] notebook
 
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# To a 3-necked flask is added
 
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## 25mL of 20 mM L-cysteine (roughly 22% L-Cysteine)
 
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## 25mL .1459 mM BSA (roughly 78% by mass)
 
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## 5mL of 100mM ZnSO<sub>4</sub> (0.5 mmol)
 
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## 5.437mL of 10.3mM MnCl<sub>2</sub> (0.056 mmol)
 
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## The pH of this mixture is adjusted to 11 with ~1M NaOH (should be on the order of 60uL)
 
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# This mixture is stirred under Ar (I'll use N<sub>2</sub>) for 30 minutes
 
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# 5.505mL of 101mM Na<sub>2</sub>S is quickly added.
 
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# The solution is stirred for 20 minutes
 
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# The solution is then aged at 50C under air for 2 hours.
 

Revision as of 13:11, 5 March 2014

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Objective

  • To start the new synthesis method




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