User:Eleni N. Kalivas/Notebook/CHEM-571/2014/03/18

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(Entry title)
Current revision (13:42, 19 March 2014) (view source)
(Table of Volumes for Large Synthesis)
 
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==Preparation of Stock Solutions==
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|''''''
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| align="center" style="background:#f0f0f0;"|'''mass in grams (g)'''
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| align="center" style="background:#f0f0f0;"|'''Final Volume (mL)'''
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| 5mM Zinc Nitrate||0.0745||50
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|-
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| 5mM Manganese Acetate||0.0123||10
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|-
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| 40mM Ammonium Acetate ||0.1541||50
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|-
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| 10uM BSA||0.033||50
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|-
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| 10mM Ammonium Hydroxide||168.92 (uL)||250
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|-
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| 5mM Sodium Sulfide||0.06||50
|}
|}

Current revision

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Objective

To begin collecting all data and based on our results refine the protocol for the rest of the semester.

Table of Volumes for Large Synthesis

80% BSA-Cysteine

% BSA 5 mM Zinc Nitrate (uL) 5mM Manganese Acetate (uL) 40 mM Ammonium Acetate (uL) 10uM BSA* (uL) 10uM 1-Thioglycerol (uL) 10mM Ammonium Hydroxide (uL) 5 mM Sodium Sulfide* (uL) Total Volume (mL)
8010800120012000960024006000012000108

80% BSA-Cysteine, half concentration

% BSA 2.5 mM Zinc Nitrate (uL) 2.5 mM Manganese Acetate (uL) 20 mM Ammonium Acetate (uL) 5 uM BSA* (uL) 5 uM L-Cysteine (uL) 5 mM Ammonium Hydroxide (uL) 2.5 mM Sodium Sulfide* (uL) Total Volume (mL)
8010800120012000960024006000012000108

80% BSA-GSH

% BSA 5 mM Zinc Nitrate (uL) 5mM Manganese Acetate (uL) 40 mM Ammonium Acetate (uL) 10uM BSA* (uL) 10uM GSH (uL) 10mM Ammonium Hydroxide (uL) 5 mM Sodium Sulfide* (uL) Total Volume (mL)
8010800120012000960024006000012000108

80% BSA-1-Thioglycerol

% BSA 5 mM Zinc Nitrate (uL) 5mM Manganese Acetate (uL) 40 mM Ammonium Acetate (uL) 10uM BSA* (uL) 10uM 1-Thioglycerol (uL) 10mM Ammonium Hydroxide (uL) 5 mM Sodium Sulfide* (uL) Total Volume (mL)
8010800120012000960024006000012000108

Preparation of Stock Solutions

' mass in grams (g) Final Volume (mL)
5mM Zinc Nitrate0.074550
5mM Manganese Acetate0.012310
40mM Ammonium Acetate 0.154150
10uM BSA0.03350
10mM Ammonium Hydroxide168.92 (uL)250
5mM Sodium Sulfide0.0650


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