User:Eleni N. Kalivas/Notebook/CHEM-571/2014/03/26: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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== | ==Objective== | ||
* | *Repeat the 78% BSA-L-Cysteine Synthesis | ||
*Continue precipitation of large volume samples | |||
==Stock Solution Prep== | |||
The solutions created for 78%BSA by mass with L-Cysteine synthesis: | |||
# 250mL of 20mM L-cysteine | |||
## FW 121.16g/mol | |||
## measure out: 0.6058g | |||
## measured: 0.6072g | |||
## actual conc: 20.05mM | |||
# 50mL of 100mM ZnSO<sub>4</sub> | |||
## FW 287.56g/mol | |||
## measure out: 1.4378g | |||
## measured: 1.4399 g | |||
## actual conc: 100.1 mM | |||
# 50mL of 10mM MnCl<sub>2</sub> | |||
## FW 197.9g/mol | |||
## measure out: 0.09895g | |||
## measured: 0.0987g | |||
## actual conc: 9.98 mM | |||
# 10mL 1M NaOH | |||
## FW 40.00g/mol | |||
## 0.4284g | |||
## Final concentration a bit over 1M check pH carefully when adjusting! | |||
# 25mL 100mM Na<sub>2</sub>S | |||
## FW 240.18g/mol | |||
## measure out: 0.600g | |||
## measured: 0.610g | |||
## actual conc: 101.6 mM | |||
# 25mL .1484 mM BSA | |||
Latest revision as of 23:53, 26 September 2017
Project name | Main project page Previous entry Next entry |
Objective
Stock Solution PrepThe solutions created for 78%BSA by mass with L-Cysteine synthesis:
|