User:Eleni N. Kalivas/Notebook/CHEM-571/2014/03/26: Difference between revisions
From OpenWetWare
(Autocreate 2014/03/26 Entry for User:Eleni_N._Kalivas/Notebook/CHEM-571) |
|||
| Line 6: | Line 6: | ||
| colspan="2"| | | colspan="2"| | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
== | ==Objective== | ||
* | *Repeat the 78% BSA-L-Cysteine Synthesis | ||
*Continue precipitation of large volume samples | |||
==Stock Solution Prep== | |||
The solutions created for 78%BSA by mass with L-Cysteine synthesis: | |||
# 250mL of 20mM L-cysteine | |||
## FW 121.16g/mol | |||
## measure out: 0.6058g | |||
## measured: 0.6072g | |||
## actual conc: 20.05mM | |||
# 50mL of 100mM ZnSO<sub>4</sub> | |||
## FW 287.56g/mol | |||
## measure out: 1.4378g | |||
## measured: 1.4399 g | |||
## actual conc: 100.1 mM | |||
# 50mL of 10mM MnCl<sub>2</sub> | |||
## FW 197.9g/mol | |||
## measure out: 0.09895g | |||
## measured: 0.0987g | |||
## actual conc: 9.98 mM | |||
# 10mL 1M NaOH | |||
## FW 40.00g/mol | |||
## 0.4284g | |||
## Final concentration a bit over 1M check pH carefully when adjusting! | |||
# 25mL 100mM Na<sub>2</sub>S | |||
## FW 240.18g/mol | |||
## measure out: 0.600g | |||
## measured: 0.610g | |||
## actual conc: 101.6 mM | |||
# 25mL .1484 mM BSA | |||
Revision as of 12:19, 15 April 2014
 Project name
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Objective
Stock Solution PrepThe solutions created for 78%BSA by mass with L-Cysteine synthesis:
| |
