User:Eleni N. Kalivas/Notebook/CHEM-571/2014/03/26

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(Autocreate 2014/03/26 Entry for User:Eleni_N._Kalivas/Notebook/CHEM-571)
Current revision (14:19, 15 April 2014) (view source)
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==Entry title==
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==Objective==
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* Insert content here...
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*Repeat the 78% BSA-L-Cysteine Synthesis
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*Continue precipitation of large volume samples
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==Stock Solution Prep==
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The solutions created for 78%BSA by mass with L-Cysteine synthesis:
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# 250mL of 20mM L-cysteine
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## FW 121.16g/mol
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## measure out: 0.6058g
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## measured: 0.6072g
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## actual conc: 20.05mM
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# 50mL of 100mM ZnSO<sub>4</sub>
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## FW 287.56g/mol
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## measure out: 1.4378g
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## measured: 1.4399 g
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## actual conc: 100.1 mM
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# 50mL of 10mM MnCl<sub>2</sub>
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## FW 197.9g/mol
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## measure out: 0.09895g
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## measured: 0.0987g
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## actual conc: 9.98 mM
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# 10mL 1M NaOH
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## FW 40.00g/mol
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## 0.4284g
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## Final concentration a bit over 1M check pH carefully when adjusting!
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# 25mL 100mM Na<sub>2</sub>S
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## FW 240.18g/mol
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## measure out: 0.600g
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## measured: 0.610g
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## actual conc: 101.6 mM
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# 25mL .1484 mM BSA
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Objective

  • Repeat the 78% BSA-L-Cysteine Synthesis
  • Continue precipitation of large volume samples


Stock Solution Prep

The solutions created for 78%BSA by mass with L-Cysteine synthesis:

  1. 250mL of 20mM L-cysteine
    1. FW 121.16g/mol
    2. measure out: 0.6058g
    3. measured: 0.6072g
    4. actual conc: 20.05mM
  2. 50mL of 100mM ZnSO4
    1. FW 287.56g/mol
    2. measure out: 1.4378g
    3. measured: 1.4399 g
    4. actual conc: 100.1 mM
  3. 50mL of 10mM MnCl2
    1. FW 197.9g/mol
    2. measure out: 0.09895g
    3. measured: 0.0987g
    4. actual conc: 9.98 mM
  4. 10mL 1M NaOH
    1. FW 40.00g/mol
    2. 0.4284g
    3. Final concentration a bit over 1M check pH carefully when adjusting!
  5. 25mL 100mM Na2S
    1. FW 240.18g/mol
    2. measure out: 0.600g
    3. measured: 0.610g
    4. actual conc: 101.6 mM
  6. 25mL .1484 mM BSA




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