User:Eleni N. Kalivas/Notebook/CHEM-571/2014/04/08: Difference between revisions

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(Autocreate 2014/04/08 Entry for User:Eleni_N._Kalivas/Notebook/CHEM-571)
 
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==Entry title==
==Objective==
* Insert content here...
# UV-vis and fluorescence of all 6 QD synthesis from 04/02/14.
# Make BSA solution 0.1459mM
# De-gas all solutions
# Synthesis using Gonzalez Method
 
*BSA made using 0.2409g in 25mL --> 0.1459mM BSA
 
==Protocol==
 
This protocol was taken from [[User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2014/02/03|Matt Harting's]] notebook, all solutions de-gassed before synthesis.
 
# To a 3-necked flask is added
## 25mL of 20 mM L-cysteine (roughly 22% L-Cysteine)
## 25mL .1459 mM BSA (roughly 78% by mass)
## 5mL of 100mM ZnSO<sub>4</sub> (0.5 mmol)
## 5.437mL of 10.3mM MnCl<sub>2</sub> (0.056 mmol)
## The pH of this mixture is adjusted to 11 with ~1M NaOH (should be on the order of 60uL)
# This mixture is stirred under Ar (I'll use N<sub>2</sub>) for 30 minutes
# 5.505mL of 101mM Na<sub>2</sub>S is quickly added.
# The solution is stirred for 20 minutes
# The solution is then aged at 50C under air for 2 hours.
 
 




Revision as of 08:55, 8 April 2014

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Objective

  1. UV-vis and fluorescence of all 6 QD synthesis from 04/02/14.
  2. Make BSA solution 0.1459mM
  3. De-gas all solutions
  4. Synthesis using Gonzalez Method
  • BSA made using 0.2409g in 25mL --> 0.1459mM BSA

Protocol

This protocol was taken from Matt Harting's notebook, all solutions de-gassed before synthesis.

  1. To a 3-necked flask is added
    1. 25mL of 20 mM L-cysteine (roughly 22% L-Cysteine)
    2. 25mL .1459 mM BSA (roughly 78% by mass)
    3. 5mL of 100mM ZnSO4 (0.5 mmol)
    4. 5.437mL of 10.3mM MnCl2 (0.056 mmol)
    5. The pH of this mixture is adjusted to 11 with ~1M NaOH (should be on the order of 60uL)
  2. This mixture is stirred under Ar (I'll use N2) for 30 minutes
  3. 5.505mL of 101mM Na2S is quickly added.
  4. The solution is stirred for 20 minutes
  5. The solution is then aged at 50C under air for 2 hours.




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